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Table 6 Effect of coenzyme Q10 supplement on the mRNA expression of antioxidant enzymes (SOD, GPX and GST) in the liver of SD rats fed 1% of cholesterol diet

From: The effects of coenzyme Q10 supplement on blood lipid indices and hepatic antioxidant defense system in SD rats fed a high cholesterol diet

Items Treatment
No Cholesterol Cholesterol (1%) Significance
No CoQ10 CoQ10 (0.5%) No CoQ10 CoQ10 (0.5%)
CON COQ CHO CHCQ Coq Cho C*C
Δ Ct 2-ΔΔCt Δ Ct 2-ΔΔCt Δ Ct 2-ΔΔCt Δ Ct 2-ΔΔCt    
SOD 7.62 ± 1.69 1 6.02 ± 1.56 3.03 7.13 ± 1.54 1.40 6.12 ± 0.54 2.83 * NS NS
GPX 3.52 ± 2.15 1 2.01 ± 1.58 2.86 3.26 ± 1.05 1.20 3.27 ± 1.08 1.20 NS NS NS
GST 14.39 ± 2.08 1 14.64 ± 2.12 0.84 12.50 ± 1.42 3.71 15.43 ± 2.62 0.48 NS NS NS
  1. Means±SD (n = 6)
  2. The values are ∆Ct, which is represented as the Ct of each target gene corrected by Ct of the control gene (β-actin)
  3. The fold difference in the relative expression of the target gene was calculated as the 2-∆∆Ct
  4. NS indicates non-significant, Coq effect of coenzyme Q10, Cho effect of cholesterol, C*C interaction effect between coenzyme Q10 and cholesterol
  5. *Indicates significant difference at p < 0.05