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Table 6 Effect of coenzyme Q10 supplement on the mRNA expression of antioxidant enzymes (SOD, GPX and GST) in the liver of SD rats fed 1% of cholesterol diet

From: The effects of coenzyme Q10 supplement on blood lipid indices and hepatic antioxidant defense system in SD rats fed a high cholesterol diet

Items

Treatment

No Cholesterol

Cholesterol (1%)

Significance

No CoQ10

CoQ10 (0.5%)

No CoQ10

CoQ10 (0.5%)

CON

COQ

CHO

CHCQ

Coq

Cho

C*C

Δ Ct

2-ΔΔCt

Δ Ct

2-ΔΔCt

Δ Ct

2-ΔΔCt

Δ Ct

2-ΔΔCt

   

SOD

7.62 ± 1.69

1

6.02 ± 1.56

3.03

7.13 ± 1.54

1.40

6.12 ± 0.54

2.83

*

NS

NS

GPX

3.52 ± 2.15

1

2.01 ± 1.58

2.86

3.26 ± 1.05

1.20

3.27 ± 1.08

1.20

NS

NS

NS

GST

14.39 ± 2.08

1

14.64 ± 2.12

0.84

12.50 ± 1.42

3.71

15.43 ± 2.62

0.48

NS

NS

NS

  1. Means±SD (n = 6)
  2. The values are ∆Ct, which is represented as the Ct of each target gene corrected by Ct of the control gene (β-actin)
  3. The fold difference in the relative expression of the target gene was calculated as the 2-∆∆Ct
  4. NS indicates non-significant, Coq effect of coenzyme Q10, Cho effect of cholesterol, C*C interaction effect between coenzyme Q10 and cholesterol
  5. *Indicates significant difference at p < 0.05