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Fig. 5 | Laboratory Animal Research

Fig. 5

From: Comparison of responsiveness to cancer development and anti-cancer drug in three different C57BL/6N stocks

Fig. 5

Determination of cisplatin-induced apoptosis in tumor tissue collected from LLC cell bearing C57BL/6N mice stocks using comparing the Bax/Bcl-2 protein ratio. Total lysates were prepared from the tumor tissues of cisplatin-treated tumor bearing C57BL/6NKorl (a), C57BL/6NA (b), and C57BL/6NB (c) mice of each group, as described in Materials and Methods. A total of 50 μg of protein per sample was immunoblotted with antibodies for each protein. Three samples were assayed in triplicate by western blotting. Data represents the means ± S.E.M of three replicates. *P < 0.05 versus LLC+Ve group

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