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Fig. 3 | Laboratory Animal Research

Fig. 3

From: Verification with the utility of an established rapid assessment of brain safety for newly developed vaccines

Fig. 3

It is a safety evaluation method of vaccine against pre-established brain parenchyma. Based on the results of our established experimental conditions according to Baek et al. [1], LPS (S. enterica-derived) and commercially available vaccines are injected intraperitoneally into 7-week-old ICR mice (1.0 mg LPS/4 ml/kg) and brain hemispheres were removed after 4 h post-injection. The mRNA is extracted from the brain, and sequentially performed real time PCR (ZO-1 and occluding). The tight junction mRNA levels of the vaccine-treated group with those of the LPS-treated group were compared and potential risk to the BBB of the vaccine is evaluated. (This conceptual diagram is shown by applying the method used by Baek et al. in [1])

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