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Fig. 1 | Laboratory Animal Research

Fig. 1

From: Four amino acids as serum biomarkers for anti-asthma effects in the ovalbumin-induced asthma mouse model treated with extract of Asparagus cochinchinensis

Fig. 1

Number of immune cells and level of OVA-specific IgE in BALF. a Experimental scheme for OVA-induced asthma model and BAW treatment. b After the collection of BALF from the lungs, total cells were separated by centrifugation, and stained with May-Giemsa solution. Total cells, eosinophils and macrophages were measured within a 1 mm2 area under a light microscope at 400× magnification. b The concentration of OVA-specific IgE was quantified in BALF by an enzyme-linked immunosorbent assay (ELISA). The minimum detectable concentration of this kit is 20.7 pg/mL. The data shown represent the means ± SD of duplicates. *, P < 0.05 compared to the Control group. #, P < 0.05 compared to the OVA+Vehicle treated group. Abbreviation: Es, Eosinophil; Mc, Macrophage

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