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Fig. 1 | Laboratory Animal Research

Fig. 1

From: Rodent models for psychiatric disorders: problems and promises

Fig. 1

Single-Cell RNA Sequencing Workflow. Brain tissue from a behavioral, pharmacologic, or genetic mouse model can be isolated and microdissected to achieve brain-region specificity [1, 2]. Next cells can be dissociated using cell-specific markers, barcoded, and droplet captured [3, 4]. Individual cells undergo RNA-sequencing and can be analyzed using differential expression to assess genes that drive differences between cell types, treatment conditions, or cell trajectories [5, 6]. A clustering analysis can be performed to identify and group cells based on gene expression markers. Single-cell trajectories can track the genetic regulation of cell-fate decisions in pseudo-time in immature, intermediate, and mature cell-types

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