Fig. 4

Microphotographs of Fto immunoreactivity in the dentate gyrus in control (a), high-fat diet-fed obese (b, HFD), and D-galactose-treated aged (c, D-gal) mice. Note that Fto immunoreactivity is mainly observed in the granule cell layer (GCL) in all groups, while Fto immunoreactivity in the HFD group is also detected in the polymorphic layer (PL). Scale bar = 50 μm. d Relative optical densities (ROD) are expressed as a percentage of the value of Fto immunoreactivity in the control in the hippocampal dentate gyrus per section (n = 5 in each group. The data are analyzed by one-way analysis of variance followed by Bonferroni’s post-hoc test. ^bp < 0.05, significantly different from the HFD group). mean values ± standard deviation