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Fig. 5 | Laboratory Animal Research

Fig. 5

From: Changes of fat-mass and obesity-associated protein expression in the hippocampus in animal models of high-fat diet-induced obesity and D-galactose-induced aging

Fig. 5

Quantitative real-time polymerase chain reaction (RT-qPCR, a) and western blot (b) analysis of Fto in the hippocampi of control, high-fat diet-fed obese (HFD), and D-galactose-treated aged (D-gal) mice. Values from RT-qPCR and western blot analysis are normalized as a ratio of the Fto and β-actin immunoblot bands in the control CA1 region and control group, respectively (n = 5 or 6 in each group, the data are analyzed by one-way analysis of variance followed by Bonferroni’s post-hoc test. ap < 0.05, significantly different from the control group; bp < 0.05, significantly different from the HFD group). The bars indicate mean values ± standard deviation

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