Table 1 PCR primer sequence for H. pylori virulence genes
| Gene name | Sequence | Tm (° C) | Reference |
|---|---|---|---|
| alpA | F: AAACCGCTCTGTGGATATGG | 55.0 | NZ_CP009259.1 |
| R: GAACTGGAAGTGCGTGTTATTG | 45.6 | ||
| cagA | F: TCACTCTTGGCGATATGGAAAT | 57.5 | |
| R: ACACAGAAGACAGAGCGTTATT | 57.7 | ||
| flaA | F: GCTAAGAGCATCAATGTGGTTTC | 58.3 | |
| R: CGGTAACATCGCGCAAATTC | 58.5 | ||
| ureA | F: AGTGGGTATTGAAGCGATGTT | 57.6 | |
| R: AAGAACAACTCACCAGGAACTAA | 57.6 |