Fig. 1

Schematic diagram showing the experiment with air pouches of mice. a Air pouch generation. Sterilized air was injected subcutaneously into the dorsum of each mouse on day 0 and day 3. At six days after the initial air injection, mice received either LPS (Ec LPS, Fn LPS or Pg LPS) or saline injection into air pouches, and sacrificed after 6 h. b Gating pictures for CD11b⁺Ly6G⁺ neutrophils and CD11b⁺Ly6G⁺RANKL⁺ neutrophils in exudates of air pouches of mice. CD11b⁺Ly6G⁺ neutrophils were analyzed in live cells gated based on SSC (granularity) and FSC (relative size). Numbers of CD11b⁺Ly6G⁺RANKL⁺ neutrophils were then analyzed. The red dot or region represents unstained sample of Ec LPS group and the blue dot or region represents stained sample of Ec LPS group. LPS, lipopolysaccharide