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Fig. 1 | Laboratory Animal Research

Fig. 1

From: Compensatory role of C3 convertase on the strain difference for C3 protein expression in FVB/N, C3H/HeN and C57BL/6N mice

Fig. 1

Expression level of mRNA, InACF and ACF for C3. a The levels of C3 transcripts in the total mRNA of liver tissue were measured by quantitative real-time (qRT)-PCR analyses using specific primers. The mRNA level of each gene was calculated based on the intensity of actin as an endogenous control. b Expression levels of InACF C3 proteins was determined by Western blot analysis using HRP-labeled anti-rabbit IgG antibody. Band intensities were determined using an imaging densitometer, and the protein expressions were calculated relative to the intensity of β-actin. c The concentration of ACF C3 was measured using the serum of three inbred mice using the Mouse Complement C3 ELISA Kit. This assay detects as low as 1.658 ng/mL. Two to three mice per group were used for the preparation of the tissue lysates and serum, and the PCR, Western blot analysis and ELISA were assayed in duplicate for each sample. Data are reported as the mean ± SD. * indicates p < 0.05 compared to the FVB/N group. Abbreviation: InACF C3, Inactive form C3; ACF C3, Active form C3

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