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Fig. 3 | Laboratory Animal Research

Fig. 3

From: Compensatory role of C3 convertase on the strain difference for C3 protein expression in FVB/N, C3H/HeN and C57BL/6N mice

Fig. 3

Level of C3 convertase activity and C2/C4 mRNA. a C3 convertase activity was measured using the serum collected from three inbred mice using a Mouse Complement C3 convertase (C3c) ELISA Kit. This assay can detect levels as low as 0.5 ng/mL. b and c The levels of C2 and C4 transcripts in the total mRNA of liver tissue were measured by quantitative real-time (qRT)-PCR analyses using specific primers. The mRNA level of each gene was calculated based on the intensity of β-actin as an endogenous control. Two to three mice per group were used for the preparation of tissue lysates and serum, and the PCR and ELISA analysis were assayed in duplicate for each sample. Data are reported as the mean ± SD. * indicates p < 0.05 when compared to the FVB/N group

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Laboratory Animal Research

ISSN: 2233-7660