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Fig. 3 | Laboratory Animal Research

Fig. 3

From: Comparison of murine retroorbital plexus and facial vein blood collection to mitigate animal ethics issues

Fig. 3

Changes in immune cell levels in the spleen. a Difference in sizes, weights, and cell numbers in mouse spleens as a function of blood collection method and researcher experience. b Immunophenotyping of RP and FV blood collection mice by flow cytometry. Dot plots show the expression of TER119, Gr-1, CD11b, CD11c, B220, CD8, and CD4 in the spleen. Graphs show proportions of cell populations in the spleen. Data are shown as means ± s.d. P values were calculated by two-tailed unpaired Student’s t-test, with blood collection method in all groups (n = 6/blood collection method). *P < 0.05, **P < 0.01, and N.S not significant

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Laboratory Animal Research

ISSN: 2233-7660