Fig. 3From: Application time and persistence of transcranial direct current stimulation (tDCS) against neuronal death resulting from transient cerebral ischemiaA The application of cathodal tDCS was performed immediately after the induction of transient cerebral ischemia. The sacrifice point is 5 days, 7 days, and 10 days after its tDCS application. B CV staining and C F-J C histofluorescence staining in gerbil hippocampal CA1 regions. (b1 and c1) In the sham group, several CV-positive cells were observed primarily in the SP layer of the hippocampal CA1 regions. However, no F-J C-positive cells were identified. (b2 and c2) In the Isch group, a few CV-positive cells and several F-J C-positive cells were observed. (b3–b5 and c3–c5) In the 5 days, 7 days and 10 days group, CV-positive cells were observed primarily in the SP layer of the hippocampal CA1 regions. A few F-J C-positive cells were observed. SO, stratum oriens; SR, stratum radiatum; SP, stratum pyramidal. Scale bar = 50 µm. (n = 7 per group)Back to article page