Fig. 5
Evolution of tumor development of animals previously immunized with non-viable MB49 tumor cells and TPG (Group B1, TPG50:MB49) or not immunized (Groups B2, TPG50, and B3, Control). After 21 days, the immunization challenge with MB49 cells (1 × 105 cells/mouse) was performed and the animals were followed up for 35 days. In case of signs of tumor development, the animals were euthanized and the bladders were removed and weighed. A Bar Graph showing mean ± SEM values of bladder weights. B Box Plot (Tukey`s test) showing the distribution of calculated tumor growth rates dividing the bladder weight by the value in days from induction to euthanasia of the animals. C Images of the animals' bladders after euthanasia, distributed according to the day of collection. Statistical analysis performed using the non-parametric Kruskal–Wallis test followed by the Dunn's multiple comparisons test, (***) p < 0.01 compared to the control group