Fig. 3
From: Chlorogenic acid modulates the ubiquitin–proteasome system in stroke animal model
Chlorogenic acid (CGA) maintains the mRNA levels of ubiquitin–proteasome system proteins in the right ischemic cortex. Image of reverse transcription-PCR products A of ubiquitin C-terminal hydrolase L1, ubiquitin thioesterase OTUB1, proteasome subunit α type 1, proteasome subunit α type 3, and proteasome subunit β type 4 in the cerebral cortex from vehicle + middle cerebral artery occlusion (MCAO), CGA + MCAO, vehicle + sham, and CGA + sham animals. MCAO induced cerebral ischemia markedly decreased the mRNA levels of the ubiquitin–proteasome system proteins while treatment with CGA inhibited the MCAO-induced decrease in the mRNA levels of these proteins. Intensities of PCR product are represented as a ratio of β-actin product intensity (B). Data (n = 5 per group) are represented as the mean ± S.E.M. *p < 0.05, **p < 0.01