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Fig. 3 | Laboratory Animal Research

Fig. 3

From: Improvement of the intestinal epithelial barrier during laxative effects of phlorotannin in loperamide-induced constipation of SD rats

Fig. 3

Expression of the AJ regulators in Lm + Pt treated SD rats. A Western blot of E-cadherin. After preparing the colon protein homogenates, the level of E-cadherin protein was measured by Western blotting analysis. B Level of p120-catenin mRNA. After preparing the total RNAs, the level of p120-catenin expression was determined by RT-qPCR analyses. The relative level of each protein band and mRNA was analyzed based on the level of β-actin. The total proteins homogenates and RNA extracts were prepared from four to six rats per group, and western blot and RT-qPCR analyses for each sample was analyzed in duplicate. All values in results are represented as the means ± standard deviation (SD). *Indicated statically significance compared to the No treated SD rats, while # indicated statically significance compared to the Lm + Vehicle treated SD rats. Abbreviation: Lm, Loperamide; Pt, Phlorotannins

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