Fig. 1

Strategy and conformation for the deletion of the Rag2 gene. A Deletion strategy for the Rag2 gene. To generate Rag2/Korl KO mice, a mixture of Cas9 protein and two sgRNA was transferred into a zygote by electroporation, as shown in Materials and Methods. The mice with a 7-nt deletion in exon 3 of the Rag2 gene were used in this study. In Rag2/J KO mice, the Rag2 gene was targeted using the Cre-LoxP system. B Expression level of Rag2 mRNA in Rag2/Korl and Rag2/J KO mice. The levels of the Rag2 transcripts were measured in the total mRNA of the bone marrow tissue by RT-qPCR using the specific primers. The mRNA levels of the Rag2 genes were calculated based on the intensity of β-actin as an endogenous control. Four to six mice per group were used to prepare the total RNA; RT-qPCR analyses were assayed in duplicate for each sample. The data are reported as the mean ± SD. *p < 0.05 compared to the WT mice