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Fig. 4 | Laboratory Animal Research

Fig. 4

From: Comparison of immunophenotypes between Rag2 knockout mice derived from two different sources

Fig. 4

FACS analysis of Th cells in the spleen of the Rag2/Korl and Rag2/J KO mice. The total cells were isolated from the spleen and stained with anti-CD3 and anti-CD4 antibodies, as described in the materials and methods. The distributions of the double negative (DN), single positive (SP), and double positive (DP) cell populations were analyzed by flow cytometry. Three to five mice per group were used to prepare and stain the total spleen cells, and the distribution of the cell population was analyzed in duplicate. The data are reported as the mean ± SD. *p < 0.05 compared to the WT mice

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