Fig. 4From: Induction of liver transplant immune tolerance in an outbred rat strain model using tacrolimusTacrolimus inhibits Th1 and Th17 cell activation. A The expression of IFN-g, IL-17, and IL-10 in the spleen from all groups at day 3 after LT was assessed by flow cytometry with gating on CD4+IFN-γ+, CD4+IL-17+, or CD4+IL-10+ cells. Data are representative of at least two independent experiments. B Spleens were examined by immunofluorescence staining with monoclonal antibodies against CD4 (green) and IL-17 (red). *P < 0.05, **P < 0.01. Original magnification, × 400Back to article page