Effects of more natural housing conditions on the muscular and skeletal characteristics of female C57BL/6J mice

Mieske, Paul; Scheinpflug, Julia; Yorgan, Timur Alexander; Brylka, Laura; Palme, Rupert; Hobbiesiefken, Ute; Preikschat, Juliane; Lewejohann, Lars; Diederich, Kai

doi:10.1186/s42826-023-00160-9

Laboratory Animal Research

Table 1 Summarized results of examined parameters and correlation analysis

From: Effects of more natural housing conditions on the muscular and skeletal characteristics of female C57BL/6J mice

Animal age	Parameter	Housing condition	Mean ± SD	CV	Correlating parameter	Corr. factor r	p Value
Days				%
340	Body weight	CON	26.8 ± g	7.7
		ENR	27.2 ± g	5.5
		SNE	29.8 ± g	8.1
	Bone density	CON	1.51 ± 0.24 g cm⁻³	15.6	Body weight	0.447	0.168
		ENR	1.69 ± 0.17 g cm⁻³	16.5		0.475	0.119
		SNE	1.72 ± 0.16 g cm⁻³	16.0		0.553	0.011
501	Body weight	CON	29.5 ± 3.4 g	11.6
		ENR	32.1 ± 4.1 g	12.7
		SNE	36.3 ± 3.2 g	8.9
	Bone density	CON	1.44 ± 0.15 g cm⁻³	10.6	Body weight	−0.027	0.937
		ENR	1.58 ± 0.19 g cm⁻³	12.0		0.391	0.234
		SNE	1.57 ± 0.23 g cm⁻³	14.4		0.460	0.047
508/510	Body weight	CON	29.8 ± 3.7 g	12.3
		ENR	31.1 ± 3.6 g	11.4
		SNE	35.2 ± 3.0 g	8.6
	Grip strength	CON	2.29 ± 0.39 N	17.0	Bone density 501 days	−0.513	0.106
		ENR	2.60 ± 0.41 N	15.6		0.795	0.003
		SNE	2.38 ± 0.35 N	14.5		0.323	0.177
664	Body weight	CON	29.4 ± 3.0 g	9.8
		ENR	31.7 ± 2.9 g	9.0
		SNE	35.0 ± 3.0 g	9.3
	Bone density	CON	1.21 ± 0.13 g cm⁻³	10.8	Body weight	0.493	0.123
		ENR	1.38 ± 0.25 g cm⁻³	17.8		0.214	0.528
		SNE	1.35 ± 0.16 g cm⁻³	12.2		0.413	0.100
	Grip strength	CON	2.34 ± 0.43 N	18.4	Bone density	0.240	0.476
		ENR	2.65 ± 0.26 N	9.8		−0.071	0.836
		SNE	2.40 ± 0.40 N	16.6		−0.196	0.451
585 — 622	Resting metabolic rate	CON	42.5 ± 7.4 ml min⁻¹ kg⁻¹	17.4	Grip str. 664 d	0.018	0.959
		ENR	36.9 ± 4.5 ml min⁻¹ kg⁻¹	12.2		0.221	0.514
		SNE	40.5 ± 3.4 ml min⁻¹ kg⁻¹	8.4		0.565	0.015
670	Body weight	CON	30.4 ± 3.1 g	10.1	RMR 585–622 d	0.326	0.329
		ENR	33.5 ± 3.1 g	9.2		0.490	0.126
		SNE	34.6 ± 3.1 g	8.9		−0.301	0.224
	Body length	CON	9.7 ± 0.3 cm	2.8	Body weight	0.838	0.001
		ENR	10.1 ± 0.2 cm	2.2		−0.182	0.592
		SNE	10.3 ± 0.2 cm	2.1		0.685	0.002
	Weight/length	CON	3.1 ± 0.3 g cm⁻¹	8.2
		ENR	3.3 ± 0.4 g cm⁻¹	10.9
		SNE	3.3 ± 0.3 g cm⁻¹	8.7
	Retroperitoneal adipose tissue weight	CON	0.057 ± 0.032 g	56.8	Body weight	0.729	0.011
		ENR	0.075 ± 0.019 g	25.3		0.874	> 0.001
		SNE	0.112 ± 0.048 g	43.1		0.539	0.021
	Periovarian adipose tissue weight	CON	0.276 ± 0.157 g	56.9	Body weight	0.535	0.090
		ENR	0.409 ± 0.182 g	44.5		0.953	> 0.001
		SNE	0.506 ± 0.236 g	46.6		0.582	0.011
	Cortical thickness	CON	164.4 ± 23.2 µm	14.1
		ENR	169.5 ± 19.7 µm	11.6
		SNE	185.5 ± 24.5 µm	13.2
	Femur length	CON	16.55 ± 0.15 mm	0.9
		ENR	16.67 ± 0.32 mm	1.9
		SNE	16.79 ± 0.28 mm	1.7
	Femur midshaft outer diameter	CON	1.76 ± 0.04 mm	2.3	Bone density 664 days	0.409	0.275
		ENR	1.77 ± 0.03 mm	1.8		0.127	0.745
		SNE	1.83 ± 0.04 mm	2.3		0.478	0.116
	Femur bone volume/tissue volume	CON	1.32 ± 0.78%	59.0
		ENR	1.78 ± 1.09%	61.1
		SNE	1.53 ± 1.10%	71.8
	Muscle weight	CON	0.123 ± 0.023 g	19.0	Body weight	0.221	0.515
					grip str. 664 d	−0.136	0.590
					RMR 585–622 d	0.313	0.348
		ENR	0.127 ± 0.021 g	16.5	Body weight	0.055	0.872
					grip str. 664 d	0.077	0.823
					RMR 585–622 d	−0.284	0.398
		SNE	0.120 ± 0.020 g	16.3	Body weight	0.435	0.071
					grip str. 664 d	0.296	0.377
					RMR 585–622 d	−0.409	0.092
	Myostatin concentration	CON	692.26 ± 132.87 pg ml⁻¹	19.2	muscle weight	0.026	0.191
		ENR	714.96 ± 107.04 pg ml⁻¹	15.0		0.711	0.178
		SNE	692.26 ± 132.87 pg ml⁻¹	19.2		−0.323	0.939
	Osteocalcin concentration	CON	4.77 ± 3.67 ng ml⁻¹	77.0	Cortical thickness	−0.180	0.642
					Cortical porosity	0.376	0.319
		ENR	4.48 ± 2.96 ng ml⁻¹	66.1	Cortical thickness	−0.016	0.969
					Cortical porosity	−0.113	0.790
		SNE	5.18.26 ± 2.79 ng ml⁻¹	53.8	Cortical thickness	−0.721	0.008
					Cortical porosity	0.685	0.014
	CTX-1 concentration	CON	32.97 ± 7.20 ng ml⁻¹	21.8	Cortical thickness	−0.470	0.240
					Cortical porosity	0.201	0.634
		ENR	31.00 ± 7.80 ng ml⁻¹	25.2	Cortical thickness	0.278	0.505
					Cortical porosity	−0.213	0.613
		SNE	24.77 ± 4.38 ng ml⁻¹	17.7	Cortical thickness	−0.562	0.057
					Cortical porosity	0.602	0.038
	FCMs	CON	2.53 ± 0.77 ng mg⁻¹	30.5
		ENR	2.73 ± 0.78 ng mg⁻¹	28.5
		SNE	1.94 ± 0.65 ng mg⁻¹	33.6
	Corticosterone (V. facialis)	CON	142.32 ± 93.33 ng ml⁻¹	65.5
		ENR	132.44 ± 81.47 ng ml⁻¹	61.5
		SNE	321.50 ± 139.66 ng ml⁻¹	43.3
	Corticosterone (perfusion)	CON	136.95 ± 90.79 ng ml⁻¹	66.3
		ENR	78.95 ± 61.60 ng ml⁻¹	78.0
		SNE	163.47 ± 102.92 ng ml⁻¹	63.0

Shown is the age of the animals at the respective time of measurement and the value of the parameter for the animals from the three housing conditions CON, ENR and SNE housing. Values are shown as the mean with the standard deviation (SD) and the coefficient of variance (CV). The housing condition showing the lowest CV per measured parameter is marked Bold. If a correlation analysis with another measured value was made, it is marked as the correlating parameter and the results are shown as the correlation factor r and the respective p value. If a correlation lead to a p value lower than 0.05, the correlation is marked underline

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ISSN: 2233-7660

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