Table 1 List of proteins that were differentially expressed between vehicle- and glutamate-treated animals
Spot no | Protein name | Accession no | Mw (kDa) | pI | Mass matched | Sequence Coverage (%) |
|---|---|---|---|---|---|---|
Protein degradation | ||||||
2 | heat shock protein 60 | P63038 | 60.96 | 5.91 | 19/45 | 41 |
13 | ubiquitin carboxy-terminal hydrolase L1 | Q7TQI3 | 31.27 | 4.85 | 11/66 | 58 |
14 | ubiquitin thiolesterase OTUB1 | B2RYG6 | 31.27 | 4.8 | 14/39 | 61 |
23 | proteasome subunit alpha type 3 | P18422 | 28.40 | 5.3 | 7/112 | 27 |
36 | proteasome subunit alpha type 2 | P17220 | 25.93 | 6.9 | 6/109 | 33 |
39 | proteasome subunit alpha type 4 | P21670 | 29.50 | 7.6 | 5/98 | 22 |
Signal transduction | ||||||
1 | dihydropyrimidinase-related protein 2 | P47942 | 62.27 | 6.0 | 24/103 | 52 |
5 | rab GDP dissociation inhibitor beta | P50399 | 50.50 | 5.9 | 9/150 | 23 |
7 | adenosylhomocysteinase | P10760 | 47.54 | 6.07 | 15/132 | 33 |
18 | protein phosphatase 2A, subunit A | P63331 | 35.60 | 5.3 | 13/110 | 45 |
24 | alpha-synuclein | P37377 | 14.50 | 4.7 | 6/134 | 43 |
30 | mitogen-activated protein kinase 1 | P63086 | 41.28 | 6.5 | 10/96 | 36 |
31 | mitogen-activated protein kinase 1 | P63086 | 41.28 | 6.5 | 10/96 | 36 |
Apoptosis | ||||||
8 | 14–3-3 Gamma | P61983 | 28.28 | 4.8 | 8/77 | 35 |
9 | 14–3-3 Zeta/delta | P63102 | 27.77 | 4.7 | 12/122 | 41 |
10 | 14–3-3 Zeta/delta | P63102 | 27.77 | 4.7 | 8/125 | 36 |
11 | 14–3-3 Beta/alpha | P35213 | 28.05 | 4.8 | 11/110 | 43 |
12 | 14–3-3 Epsilon | P62260 | 29.17 | 4.6 | ||
Anti-oxidant | ||||||
15 | thioredoxin | Q920J4 | 32.23 | 4.84 | 8/87 | 42 |
35 | peroxiredoxin-5 | Q9R063 | 22.17 | 8.9 | 9/114 | 46 |
Calcium binding protein | ||||||
25 | hippocalcin | P62749 | 22.32 | 5.3 | 10/102 | 52 |
26 | parvalbumin alpha | P02625 | 11.92 | 5 | 5/103 | 40 |
Metabolism | ||||||
6 | succinyl-CoA ligase subunit beta, mitochondrial | Q9Z2I9 | 50.11 | 6.6 | 6/104 | 12 |
20 | isocitrate dehydrogenase (NAD+) subunit alpha | Q99NA5 | 39.59 | 6.47 | 8/93 | 31 |
27 | pyruvate kinase isoenzyme M1/M2 | P11980 | 57.82 | 6.63 | 19/80 | 42 |
32 | glyceraldehyde-3-phosphate dehydrogenase | P04797 | 35.83 | 8.14 | 16/106 | 46 |
33 | glyceraldehyde-3-phosphate dehydrogenase | P04797 | 35.83 | 8.14 | 21/94 | 62 |
34 | nucleoside diphosphate kinase B | P19804 | 17.28 | 6.9 | 8/86 | 49 |
37 | adenylate kinase isoenzyme 1 | P39069 | 21.58 | 7.7 | 6/113 | 34 |
38 | triosephosphate isomerase | P48500 | 26.85 | 6.9 | 11/103 | 51 |
40 | vacuolar proton pump subunit E1 | Q6PCU2 | 26.13 | 8.4 | 11/101 | 40 |
41 | nucleoside diphosphate kinase B | P19804 | 17.28 | 6.9 | 8/86 | 49 |
Other & Unknown | ||||||
4 | prolactin-8A5 isoform XI | P33579 | 27.27 | 5.47 | 7/97 | 22 |
16 | mu-crystallin | Q9QYU4 | 33.53 | 5.34 | 9/86 | 24 |
17 | mu-crystallin | Q9QYU4 | 33.55 | 5.3 | 6/114 | 21 |
3 | unknown | |||||
19 | unkonwn | |||||
21 | unknown | |||||
28 | unknown | |||||
29 | unknown | |||||