Articles

Zebrafish toxicological screening could aid Leishmaniosis drug discovery

Recently a screen from a library of 1.8 million compounds identified in vitro a potent activity of the 2-aminobenzimidazoles series against Leishmania infantum, the etiological agent responsible by over 20.000 de...

Authors: Hirla Costa Silva Fukushima, Ricardo Lacava Bailone, Tatiana Corrêa, Helena Janke, Luís Kluwe De Aguiar, Princia Grejo Setti and Ricardo Carneiro Borra

Transgenic fluorescent zebrafish lines that have revolutionized biomedical research

Since its debut in the biomedical research fields in 1981, zebrafish have been used as a vertebrate model organism in more than 40,000 biomedical research studies. Especially useful are zebrafish lines express...

Authors: Chong Pyo Choe, Seok-Yong Choi, Yun Kee, Min Jung Kim, Seok-Hyung Kim, Yoonsung Lee, Hae-Chul Park and Hyunju Ro

A comparative review on heart ion channels, action potentials and electrocardiogram in rodents and human: extrapolation of experimental insights to clinic

Electrocardiogram (ECG) is a non-invasive valuable diagnostic tool that is used in clinics for investigation and monitoring of heart electrical rhythm/conduction, ischemia/injury of heart, electrolyte disturba...

Authors: Siyavash Joukar

Increases in GFAP immunoreactive astrocytes in the cerebellar molecular layer of young adult CBA/J mice

CBA/J mice are standard experimental animals in auditory studies, and age-related changes in auditory pathways are well documented. However, changes in locomotion-related brain regions have not been systematic...

Authors: Cheryl Tyszkiewicz, Ingrid D Pardo, Hayley N Ritenour, Chang-Ning Liu and Chris Somps

Experimental animal models for diabetes and its related complications—a review

Diabetes mellitus, a very common and multifaceted metabolic disorder is considered as one of the fastest growing public health problems in the world. It is characterized by hyperglycemia, a condition with high...

Authors: Chidhambara Priya Dharshini Kottaisamy, Divya S. Raj, V. Prasanth Kumar and Umamaheswari Sankaran

Correction to: Lipopolysaccharides of Fusobacterium nucleatum and Porphyromonas gingivalis increase RANKL-expressing neutrophils in air pouches of mice

Authors: Ae Ri Kim, Yun Kyong Lim, Joong-Ki Kook, Eun-Jung Bak and Yun-Jung Yoo

Comparison of intrinsic exercise capacity and response to acute exercise in ICR (Institute of Cancer Research) mice derived from three different lineages

As a laboratory animal resource, the ICR mouse is commonly used in a variety of research fields. However, information on differences in exercise-related characteristics in ICR mice derived from different linea...

Authors: Dong-Joo Hwang, Ki-Chun Kwon, Dong-Hun Choi, Hyun-Keun Song, Kil-Soo Kim, Young-Suk Jung, Dae-Youn Hwang and Joon-Yong Cho

Establishment of particulate matter-induced lung injury model in mouse

Particulate matter (PM) is one of the principal causes of human respiratory disabilities resulting from air pollution. Animal models have been applied to discover preventive and therapeutic drugs for lung dise...

Authors: Se Yong Park, Kyu Sup An, Buhyun Lee, Ju-Hee Kang, Hyun Jin Jung, Min Woo Kim, Hyeon Yeol Ryu, Kyu-Suk Shim, Ki Taek Nam, Yeo Sung Yoon and Seung Hyun Oh

Induction of colorectal carcinogenesis in the C57BL/6J and A/J mouse strains with a reduced DSS dose in the AOM/DSS model

Colorectal cancer (CRC) is one of the most frequently diagnosed cancers worldwide and thus mouse models of CRC are of significant value to study the pathogenesis. The Azoxymethane/Dextran sulfate sodium (AOM/D...

Authors: Henriette Arnesen, Mette Helen Bjørge Müller, Mona Aleksandersen, Gunn Charlotte Østby, Harald Carlsen, Jan Erik Paulsen and Preben Boysen

Working with laboratory rodents in Spain: a survey on welfare and wellbeing

Replacement, reduction and refinement, the 3R principles, provide a framework to minimize the use and suffering of animals in science. In this context, we aimed to determine the actual perception that individu...

Authors: Olatz Goñi-Balentziaga, Iván Ortega-Saez, Sergi Vila and Garikoitz Azkona

Acute and 28-days subacute toxicity studies of Gαq-RGS2 signaling inhibitor

The aim of study was to evaluate the single oral dose and 28 day repeated oral administration toxicity profile of the synthetic compound Gαq-RGS2 signaling inhibitor, (1-(5-chloro-2-hydroxyphenyl)-3-(4-(triflu...

Authors: Jayesh V. Beladiya and Anita A. Mehta

Hypothermic treatment reduces matrix metalloproteinase-9 expression and damage in the liver following asphyxial cardiac arrest in rats

Hypothermic treatment is known to protect organs against cardiac arrest (CA) and improves survival rate. However, few studies have evaluated the effects of hypothermia on CA-induced liver damages. This study w...

Authors: Donghwi Kim, Bora Kim, Hyejin Sim, Tae-Kyeong Lee, Hyun-Jin Tae, Jae-Chul Lee, Joon Ha Park, Jun Hwi Cho, Moo-Ho Won, Yoonsoo Park and Ji Hyeon Ahn

A comparative study on intraocular pressure under various anesthetics in cynomolgus monkeys (Macaca fascicularis)

Nonhuman primates (NHPs) are superior model for ocular research due to its morphological and physiological similarities with humans. Thus, the effect of four different anesthetic combinations [ketamine (10 mg/...

Authors: Hong-Soo Lee, Da-Hee Kim, Sung-Hwan Kim, Min-Sung Kang and Han Na Suh

Intratracheal inoculation of human varicella zoster virus (VZV; MAV strain) vaccine successfully induced VZV IgG antibodies in rhesus monkeys

The objective of this study was to investigate whether the use of live attenuated varicella zoster virus (VZV) MAV vaccination can efficiently induce VZV antibody production in naive rhesus monkeys as an appro...

Authors: Jong-Min Kim and Chung-Gyu Park

A guide to reducing adverse outcomes in rabbit models of sciatic nerve injury

Peripheral nerve damage can have debilitating consequences. Rabbit sciatic nerve transection models allow the effective evaluation of surgical repair strategies for large nerve gaps. Despite advantages in size...

Authors: Elisabeth Orozco, Koichi Masuda and Sameer B. Shah

Comparison of murine retroorbital plexus and facial vein blood collection to mitigate animal ethics issues

Blood collection is an important procedure used in animal experiments. Blood collection methods that reduce pain, injury, and stress in experimental animals are important with regard to animal ethics. Various ...

Authors: Eun Jung Jo, Eunjin Bae, Jeong-Hwan Yoon, Ji Yeon Kim and Jin Soo Han

Comparison of age‐dependent alterations in thioredoxin 2 and thioredoxin reductase 2 expressions in hippocampi between mice and rats

Aging is one of major causes triggering neurophysiological changes in many brain substructures, including the hippocampus, which has a major role in learning and memory. Thioredoxin (Trx) is a class of small r...

Authors: Yeon Ho Yoo, Dae Won Kim, Bai Hui Chen, Hyejin Sim, Bora Kim, Jae-Chul Lee, Ji Hyeon Ahn, Yoonsoo Park, Jun Hwi Cho, Il Jun Kang, Moo-Ho Won and Tae-Kyeong Lee

Sex-dependent liver cancer xenograft models for predicting clinical data in the evaluation of anticancer drugs

The incidence and mortality of liver cancer show a great difference between the sexes. We established sex-dependent liver cancer xenograft models and investigated whether such sex-dependent models could be use...

Authors: Sungryong Oh and Joohee Jung

Quercetin attenuates the reduction of parvalbumin in middle cerebral artery occlusion animal model

Calcium is a critical factor involved in modulation of essential cellular functions. Parvalbumin is a calcium buffering protein that regulates intracellular calcium concentrations. It prevents rises in calcium...

Authors: Dong-Ju Park, Ju-Bin Kang, Fawad-Ali Shah and Phil-Ok Koh

Inflammatory responses of C57BL/6NKorl mice to dextran sulfate sodium-induced colitis: comparison between three C57BL/6 N sub-strains

Inflammatory bowel disease (IBD), including both Crohn’s disease and ulcerative colitis, are chronic human diseases that are challenging to cure and are often unable to be resolved. The inbred mouse strain C57...

Authors: Sou Hyun Kim, Doyoung Kwon, Seung Won Son, Tae Bin Jeong, Seunghyun Lee, Jae-Hwan Kwak, Joon-Yong Cho, Dae Youn Hwang, Min-Soo Seo, Kil Soo Kim and Young-Suk Jung

Establishment and application of information resource of mutant mice in RIKEN BioResource Research Center

Online databases are crucial infrastructures to facilitate the wide effective and efficient use of mouse mutant resources in life sciences. The number and types of mouse resources have been rapidly growing due...

Authors: Hiroshi Masuya, Daiki Usuda, Hatsumi Nakata, Naomi Yuhara, Keiko Kurihara, Yuri Namiki, Shigeru Iwase, Toyoyuki Takada, Nobuhiko Tanaka, Kenta Suzuki, Yuki Yamagata, Norio Kobayashi, Atsushi Yoshiki and Tatsuya Kushida

Tail suspension is useful as a sarcopenia model in rats

Sarcopenia promotes skeletal muscle atrophy and exhibits a high mortality rate. Its elucidation is of the highest clinical importance, but an animal experimental model remains controversial. In this study, we ...

Authors: Akira Nemoto and Toru Goyagi

Comparison of response to LPS-induced sepsis in three DBA/2 stocks derived from different sources

Sepsis, one of the most fatal diseases in the world, is known to culminate in multiple organ failure due to an uncontrolled inflammatory response. Hence, the use of animal models in sepsis research is very imp...

Authors: Ji Won Park, Su Jin Lee, Ji Eun Kim, Mi Ju Kang, Su Ji Bae, Yun Ju Choi, Jeong Eun Gong, Kil Soo Kim, Young-Suk Jung, Joon-Yong Cho, Yeon Shik Choi, Dae Youn Hwang and Hyun Keun Song

Lipopolysaccharides of Fusobacterium nucleatum and Porphyromonas gingivalis increase RANKL-expressing neutrophils in air pouches of mice

Increases of neutrophils and osteoclasts are pathological changes of periodontitis. RANKL is an osteoclast differentiation factor. The effect of periodontopathogen LPS on RANKL-expressing neutrophils has not b...

Authors: Ae Ri Kim, Yun Kyong Lim, Joong-Ki Kook, Eun-Jung Bak and Yun-Jung Yoo

A review of studies on Oryzias dancena

Oryzias dancena (Beloniformes; Teleostei), is a euryhaline teleost that mainly inhabits the brackish or freshwater of river mouths and estuaries around the Bay of Bengal and the Malay Peninsula. It also has a sho...

Authors: In-Seok Park

Nonhuman primate model in mammary gland biology and neoplasia research

Research on breast cancer pathogenesis, prevention and drug development remains an important field as this disease is still one of the leading causes of cancer death worldwide. Nonhuman primates, particularly ...

Authors: Fitriya N. Dewi and J. Mark Cline

Zootechnical data analysis in a breeding animal facility: tracing the patterns of mouse production

With the enactment of the Brazilian Law Arouca 11,794/2008 and Decree 6.899/2009, there has been an urgent need for changes in the processes related to animal experimentation in Brazil; in particular, there is...

Authors: Eloiza K. G. D. Ferreira, Giovanny A. C. A. Mazzarotto and Guilherme F. Silveira

The ‘R’ principles in laboratory animal experiments

Since the Three Rs of replacement, reduction and refinement was proposed by Russel and Birch in 1959, researchers have a moral duty to minimize harm to animals. Even though animal experiments are performed by ...

Authors: Kook Hyun Lee, Dong Won Lee and Byeong Chul Kang

Comparative analysis of immune related genes between domestic pig and germ-free minipig

Recently, minipig has been considered as an animal model that is appropriate for human disease model to study toxicology, pharmacology, and xenotransplantation. Nevertheless, minipigs are bred in various envir...

Authors: Ju Young Lee, Sang Eun Kim, Hoon Taek Lee and Jeong Ho Hwang

Extensive variability in the composition of immune infiltrate in different mouse models of cancer

Mouse models are invaluable tools for cancer immunology research. However, there are differences in the immune response to the tumour depending on the model used, and these differences are not often characteri...

Authors: Virginia Niemi, Douglas Gaskarth and Roslyn A. Kemp

Evaluation of skin phototoxicity of transdermally administered pharmaceuticals in Sprague-Dawley rats

Some drugs cause phototoxicity in humans when exposed to light, thus there is a need for an in vivo phototoxicity test to evaluate them. However, an in vivo phototoxicity test method to evaluate this has not b...

Authors: Nam Hee Youn, Eun Ji Kim, Jung-Sun Yi, Joo Hwan Kim, Ye-Jin Cho, Ki Taek Nam, Ki Sook Park and Jong Kwon Lee

Impaired liver regeneration and lipid homeostasis in CCl₄ treated WDR13 deficient mice

WDR13 - a WD repeat protein, is abundant in pancreas, liver, ovary and testis. Absence of this protein in mice has been seen to be associated with pancreatic β-cell proliferation, hyperinsulinemia and age depe...

Authors: Arun Prakash Mishra, Archana B. Siva, Chandrashekaran Gurunathan, Y. Komala and B. Jyothi Lakshmi

Anti-Helicobacter pylori activity of acomplex mixture of Lactobacillus paracasei HP7 including the extract of Perilla frutescens var. acuta and Glycyrrhiza glabra

The effect of standard therapeutic strategies on Helicobacter pylori infection is diminished over time owing to the emergence of drug resistant strains. In this study, we would like to confirm the enhanced effect...

Authors: Hyun-A Lee, Joo-Yun Kim, Jisoo Kim, Bora Nam and Okjin Kim

A comparative study of the phenotype with kainic acid-induced seizure in DBA/2 mice from three different sources

The kainic acid-induced seizure mouse model is widely used in epilepsy research. In this study, we applied kainic acid to the subcutaneous injections of three different sources of DBA/2 mice to compare and eva...

Authors: Kyung-Ku Kang, Young-In Kim, Min-Soo Seo, Soo-Eun Sung, Joo-Hee Choi, Sijoon Lee, Young-Suk Jung, Joon Young Cho, Dae Youn Hwang, Sang-Joon Park and Kil Soo Kim

Pathogenicity of severe fever with thrombocytopenia syndrome virus in mice regulated in type I interferon signaling

Severe fever with thrombocytopenia syndrome (SFTS) is an emerging zoonotic disease, which causes high fever, thrombocytopenia, and death in humans and animals in East Asian countries. The pathogenicity of SFTS...

Authors: Seok-Chan Park, Jun Young Park, Jin Young Choi, Sung-Geun Lee, Seong Kug Eo, Jae-Ku Oem, Dong-Seob Tark, Myungjo You, Do-Hyeon Yu, Joon-Seok Chae and Bumseok Kim

Refining drug administration in a murine model of acute infection with Trypanosoma cruzi

In animal research, “refinement” refers to modifications of husbandry or experimental procedures to enhance animal well-being and minimize or eliminate pain and distress. Evaluation of drug efficacy in mice mo...

Authors: Julián Ernesto Nicolás Gulin, Margarita Bisio and Facundo García-Bournissen

Unexpected effects of ivermectin and selamectin on inducible Cre^ER activity in mice

Anti-parasitics are frequently used in research animal facilities to treat a multitude of common infections, with pinworms and fur mites being amongst the most common. Ivermectin and selamectin are common oral...

Authors: Peter A. Kropp, Gabrielle V. Rushing, Asa A. Brockman, Erin N. Z. Yu, Rebecca A. Ihrie and Maureen Gannon

Correction to: Influence of three BALB/c substrain backgrounds on the skin tumor induction efficacy to DMBA and TPA cotreatment

An amendment to this paper has been published and can be accessed via the original article.

Authors: Mi Ju Kang, Jeong Eun Gong, Ji Eun Kim, Hyeon Jun Choi, Su Ji Bae, Yun Ju Choi, Su Jin Lee, Min-Soo Seo, Kil Soo Kim, Young-Suk Jung, Joon-Yong Cho, Yong Lim and Dae Youn Hwang

Decrease of protein phosphatase 2A subunit B by glutamate exposure in the cerebral cortex of neonatal rats

Glutamate induces neurotoxicity during brain development, causing nerve damage. Protein phosphatase 2A (PP2A) is a type of serine/threonine phosphatase that regulates various biological functions. Among the PP...

Authors: Ju-Bin Kang, Dong-Ju Park, Hyun-Kyoung Son and Phil-Ok Koh

Cryopreservation of mouse resources

The cryopreservation of sperm and embryos is useful to efficiently archive valuable resources of genetically engineered mice. Till date, more than 60,000 strains of genetically engineered mice have been archiv...

Authors: Toru Takeo, Satohiro Nakao, Yoshiko Nakagawa, Jorge M. Sztein and Naomi Nakagata

Baicalin alleviates lipopolysaccharide-induced neuroglial activation and inflammatory factors activation in hippocampus of adult mice

Baicalin is a natural flavonoid that exerts a variety of pharmaceutical effects such as anti-inflammatory and antioxidant. Lipopolysaccharide (LPS) is an endotoxin that releases inflammatory cytokines and indu...

Authors: Murad-Ali Shah, Dong-Ju Park, Ju-Bin Kang, Myeong-Ok Kim and Phil-Ok Koh

Tamoxifen-inducible cardiac-specific Cre transgenic mouse using VIPR2 intron

Genetically engineered mouse models through gene deletion are useful tools for analyzing gene function. To delete a gene in a certain tissue temporally, tissue-specific and tamoxifen-inducible Cre transgenic m...

Authors: Hyun Jung Chin, So-young Lee and Daekee Lee

Influence of three BALB/c substrain backgrounds on the skin tumor induction efficacy to DMBA and TPA cotreatment

Differences in responsiveness of BALB/c substrains have been investigated in various fields, including diabetes induction, corpus callosum deficiency, virus-induced demyelinating disease, aggressive behavior a...

Authors: Mi Ju Kang, Jeong Eun Gong, Ji Eun Kim, Hyeon Jun Choi, Su Ji Bae, Yun Ju Choi, Su Jin Lee, Min-Soo Seo, Kil Soo Kim, Young-Suk Jung, Joon-Yong Cho, Yong Lim and Dae Youn Hwang

Comparative analysis of restraint stress-induced depressive-like phenotypes in C57BL/6N mice derived from three different sources

C57BL/6NKorl mice are a novel mouse stock recently developed by the National Institute of Food and Drug Safety Evaluation in Korea. Extensive research into the nature of C57BL/6NKorl mice is being conducted. H...

Authors: Dong-Joo Hwang, Ki-Chun Kwon, Dae-Youn Hwang, Min-Soo Seo, Kil-Soo Kim, Young-Suk Jung and Joon-Yong Cho

High fat diet accelerates and exacerbates microgliosis and neuronal damage/death in the somatosensory cortex after transient forebrain ischemia in gerbils

Obesity has been known as an independent risk factor for stroke. Effects of high-fat diet (HFD)-induced obesity on neuronal damage in the somatosensory cortex of animal models of cerebral ischemia have not bee...

Authors: Won Joo Seo, Ji Hyeon Ahn, Tae-Kyeong Lee, Bora Kim, Jae-Chul Lee, Joon Ha Park, Yeon Ho Yoo, Myoung Cheol Shin, Jun Hwi Cho, Moo-Ho Won and Yoonsoo Park

Two base pair deletion in IL2 receptor γ gene in NOD/SCID mice induces a highly severe immunodeficiency

Genome editing has recently emerged as a powerful tool for generating mutant mice. Small deletions of nucleotides in the target genes are frequently found in CRISPR/Cas9 mediated mutant mice. However, there ar...

Authors: Inseon Bak, Doo-Jin Kim, Hyoung-Chin Kim, Hye-Jun Shin, Eunhye Yu, Kyeong-Won Yoo and Dae-Yeul Yu

Quisqualis indica extract ameliorates low urinary tract symptoms in testosterone propionate-induced benign prostatic hyperplasia rats

Benign prostate hyperplasia (BPH) is a common disease in old-age males, accounting for approximately 77% of morbidity within the age range of 40 to 70 years. It has been shown that morbidity increases with soc...

Authors: Dae-geon Kim, Hyo-Jeong Kwon, Jong-Hwan Lim, Joo-heon Kim and Kyu Pil Lee

Early high-fat feeding improves histone modifications of skeletal muscle at middle-age in mice

The purpose of the present study was to investigate how the effects of high-fat diet feeding on the skeletal muscle persisted during aging using mice. Post-weaned male mice were fed a high-fat diet between 1- ...

Authors: Toshihiro Yoshie, Chiharu Saito and Fuminori Kawano

Brain Factor-7® improves learning and memory deficits and attenuates ischemic brain damage by reduction of ROS generation in stroke in vivo and in vitro

Brain Factor-7® (BF-7), silk fibroin peptide, is known to be effective in improvement of memory and learning ability. In this study, the effects of BF-7 (10 mg/kg, p.o., pre-treatment for 7 days and post-treat...

Authors: Yoohun Noh, Ji Hyeon Ahn, Ji-Won Lee, Junkee Hong, Tae-Kyeong Lee, Bora Kim, Sung-Su Kim and Moo-Ho Won

Study of ethion and lipopolysaccharide interaction on lung in a mouse model

Ethion is an organophosphate used commonly in India despite being banned in many other countries. The present study was designed to study the interaction of ethion and lipopolysaccharide (LPS) together on lung...

Authors: Geetika Verma and R. S. Sethi