Articles

Characterization and applications of chimeric mice with humanized livers for preclinical drug development

We have succeeded in stable mass production of chimeric PXB-mice, whose liver is repopulated by human hepatocytes at a ratio of more than 70%, and we are providing these mice to academia and pharmaceutical com...

Authors: Chise Tateno and Yuha Kojima

Evaluation of factors related to Anaesthesia-induced Lens opacity in experimental mice

To investigate conditions that cause temporal lens opacity, we tested chemical and physical factors, such as anaesthesia dose, ocular surface dryness, and infrared (IR) light exposure in anaesthetised C57BL/6 ...

Authors: Hun Lee, Hong Kyung Kim, Hae Sol Shin, Soo Jung Han, Sangchul Yoon, Je Kyung Seong and Kyoung Yul Seo

Four amino acids as serum biomarkers for anti-asthma effects in the ovalbumin-induced asthma mouse model treated with extract of Asparagus cochinchinensis

The butanol extract of Asparagus cochinchinensis roots fermented with Weissella cibaria (BAW) effectively prevents inflammation and remodeling of airway in the ovalbumin (OVA)-induced asthma model. To characteriz...

Authors: Jun Young Choi, So Hyun Kim, Ji Eun Kim, Ji Won Park, Mi Ju Kang, Hyeon Jun Choi, Su Ji Bae, Jae Ho Lee, Young-Suk Jung and Dae Youn Hwang

Chronic oral administration of Passiflora incarnata extract has no abnormal effects on metabolic and behavioral parameters in mice, except to induce sleep

Although the number of prescriptions and dependence on sleeping pills are increasing, the associations with unexpected abnormal behaviors and metabolic diseases caused by the overuse of sleeping pills are not ...

Authors: Gwang-Ho Kim and Sun Shin Yi

Inhibition of STAT3 signaling induces apoptosis and suppresses growth of lung cancer: good and bad

Signal transducer and activator of transcription 3 (STAT3) modulates a variety of genes involved in the regulation of critical functions, including cell proliferation, differentiation, apoptosis, angiogenesis,...

Authors: Ju-Hee Kang, Yeong-Su Jang, Ha Jung Lee, Chang-Yong Lee, Dong Yun Shin and Seung Hyun Oh

Investigation of the effect of coffee on body weight, serum glucose, uric acid and lipid profile levels in male albino Wistar rats feeding on high-fructose diet

Coffee is one of the most commonly consumed beverages in the worldwide and is assumed to have protective effects against metabolic syndrome. The present study was aimed at investigating the effect of coffee on...

Authors: Teka Obsa Feyisa, Daniel Seifu Melka, Menakath Menon, Wajana Lako Labisso and Mezgebu Legesse Habte

An improvement in acute wound healing in rats by the synergistic effect of photobiomodulation and arginine

In this probe, at first we examined the best route and dosage of arginine administration on wound healing in an excisional wound model in rats. Next, we intend to assess the impact of photobiomodulation (PBM) ...

Authors: Atarodsadat Mostafavinia, Mohammad Bidram, Amirhossein Gomi Avili, Mohammadamin Mahmanzar, Seyed Ali Karimifard, Ensieh Sajadi, Abdollah Amini, Mahsa Hadipour Jahromy, Seyed Kamran Ghoreishi, Sufan Chien and Mohammad Bayat

Antitumor effect of TW-37, a BH3 mimetic in human oral cancer

TW-37 is a small molecule B cell lymphoma-2 (Bcl-2) homology 3 mimetic with potential anticancer activities. However, the in vivo anti-cancer effect of TW-37 in human oral cancer has not been properly studied ...

Authors: Chi-Hyun Ahn, Won Woo Lee, Yun Chan Jung, Ji-Ae Shin, Kyoung-Ok Hong, Sujung Choi, Neeti Swarup, Jihoon Kim, Min-Hye Ahn, Minjung Jung, Sung-Dae Cho and Bohwan Jin

Implication of Porphyromonas gingivalis in colitis and homeostasis of intestinal epithelium

Emerging evidences have reported that periodontitis can be a risk factor for the pathogenesis of various systemic diseases. Porphyromonas gingivalis (Pg), one of the crucial pathogens in chronic periodontitis, ha...

Authors: Yoojin Seo, Su-Jeong Oh, Ji-Su Ahn, Ye Young Shin, Ji Won Yang and Hyung-Sik Kim

Verification with the utility of an established rapid assessment of brain safety for newly developed vaccines

In the twenty-first century, high contagious infectious diseases such as SARS (Severe Acute Respiratory Syndrome), MERS (Middle East Respiratory Syndrome), FMD (Foot-and-Mouth Disease) and AI (Avian Influenza)...

Authors: Gwang-Ho Kim and Sun Shin Yi

Identification of proteins differentially expressed by glutamate treatment in cerebral cortex of neonatal rats

Glutamate leads to neuronal cell damage by generating neurotoxicity during brain development. The objective of this study is to identify proteins that differently expressed by glutamate treatment in neonatal c...

Authors: Ju-Bin Kang, Dong-Ju Park and Phil-Ok Koh

TarGo: network based target gene selection system for human disease related mouse models

Genetically engineered mouse models are used in high-throughput phenotyping screens to understand genotype-phenotype associations and their relevance to human diseases. However, not all mutant mouse lines with...

Authors: Daejin Hyung, Ann-Marie Mallon, Dong Soo Kyung, Soo Young Cho and Je Kyung Seong

Involvement of immune responses in pulmonary arterial hypertension; lessons from rodent models

Pulmonary hypertension (PH) is a pathological state with sustained elevation of pulmonary artery (PA) pressure. Since the pathogenesis of PH is mostly irreversible, the disease often comes up with poor prognos...

Authors: Kibyeong Kim and Jae-Hoon Choi

Heat shock protein 70 increases cell proliferation, neuroblast differentiation, and the phosphorylation of CREB in the hippocampus

In the present study, we investigated the effects of heat shock protein 70 (HSP70) on novel object recognition, cell proliferation, and neuroblast differentiation in the hippocampus. To facilitate penetration ...

Authors: Hyun Jung Kwon, Woosuk Kim, Hyo Young Jung, Min Soo Kang, Jong Whi Kim, Kyu Ri Hahn, Dae Young Yoo, Yeo Sung Yoon, In Koo Hwang and Dae Won Kim

Age-dependent changes of p53 and p63 immunoreactivities in the mouse hippocampus

P53 and its family member p63 play important roles in cellular senescence and organismal aging. In this study, p53 and p63 immunoreactivity were examined in the hippocampus of young, adult and aged mice by usi...

Authors: Tae-Kyeong Lee, Young Eun Park, Cheol Woo Park, Bora Kim, Jae-Chul Lee, Joon Ha Park, Hyang-Ah Lee, Moo-Ho Won and Ji Hyeon Ahn

Lipopolysaccharide induces neuroglia activation and NF-κB activation in cerebral cortex of adult mice

Lipopolysaccharide (LPS) acts as an endotoxin, releases inflammatory cytokines, and promotes an inflammatory response in various tissues. This study investigated whether LPS modulates neuroglia activation and ...

Authors: Ju-Bin Kang, Dong-Ju Park, Murad-Ali Shah, Myeong-Ok Kim and Phil-Ok Koh

Resveratrol modulates the Akt/GSK-3β signaling pathway in a middle cerebral artery occlusion animal model

Cerebral ischemia is a major cause of neurodegenerative disease. It induces neuronal vulnerability and susceptibility, and leads to neuronal cell death. Resveratrol is a polyphenolic compound that acts as an a...

Authors: Dong-Ju Park, Ju-Bin Kang, Fawad-Ali Shah and Phil-Ok Koh

Comparison of responsiveness to cancer development and anti-cancer drug in three different C57BL/6N stocks

In our efforts to understand the systemic features of tumors, the importance of animal models is increasing due to the recent growth in the development of immunotherapy and targeted therapies. This has resulte...

Authors: Mi Ju Kang, Ji Eun Kim, Ji Won Park, Hyeon Jun Choi, Su Ji Bae, Kil Soo Kim, Young-Suk Jung, Joon-Yong Cho, Dae Youn Hwang and Hyun Keun Song

Comparison of toxic responses to acetaminophen challenge in ICR mice originating from different sources

Acetaminophen (APAP) is the most common antipyretic analgesic worldwide. However, APAP overdose causes severe liver injury, especially centrilobular necrosis, in humans and experimental animals. At therapeutic...

Authors: Tae Bin Jeong, Joung-Hee Kim, Sou Hyun Kim, Seunghyun Lee, Seung Won Son, Yong Lim, Joon-Yong Cho, Dae Youn Hwang, Kil Soo Kim, Jae-Hwan Kwak and Young-Suk Jung

Comparative study of liver injury induced by high-fat methionine- and choline-deficient diet in ICR mice originating from three different sources

Non-alcoholic fatty liver disease (NAFLD) is the leading cause of chronic liver disease worldwide. It is characterized by the accumulation of lipids without alcohol intake and often progresses to non-alcoholic...

Authors: Seunghyun Lee, Jae-Hwan Kwak, Sou Hyun Kim, Tae Bin Jeong, Seung Won Son, Joung-Hee Kim, Yong Lim, Joon-Yong Cho, Dae Youn Hwang, Kil Soo Kim and Young-Suk Jung

Correlation between synthesis of α₂-macroglobulin as acute phase protein and degree of hepatopathy in rats

The degree of hepatopathy affecting the synthesis of α₂-macroglobulin (α2M) as an acute phase protein in rats was investigated. Hepatopathy was induced in Sprague-Dawley rats by intravenous administration of gala...

Authors: Reina Ito and Takashi Kuribayashi

The effects of coenzyme Q₁₀ supplement on blood lipid indices and hepatic antioxidant defense system in SD rats fed a high cholesterol diet

A total of 24 SD rats were allotted to four treatment groups such as the control (CON), 1% of cholesterol diet (CHO), 0.5% of coenzyme Q₁₀ (COQ) and 1% of cholesterol plus 0.5% of coenzyme Q₁₀ (CHCQ) groups to de...

Authors: Ha-Na Kim, Dong-Gyung Jeon, Yong Lim and In-Surk Jang

RbAp48 expression and neuronal damage in the gerbil hippocampus following 5 min of transient ischemia

Histone-binding protein RbAp48 has been known to be involved in histone acetylation, and epigenetic alterations of histone modifications are closely associated with the pathogenesis of ischemic reperfusion inj...

Authors: Joon Ha Park, Tae-Kyeong Lee, Dae Won Kim, Cheol Woo Park, Young Eun Park, Bora Kim, Jae-Chul Lee, Hyang-Ah Lee, Moo-Ho Won and Ji Hyeon Ahn

Comparison study of the response with botulinum toxin muscle injection in the ICR mice from three different sources

Botulinum-toxin A (BoNT/A) is a widely used not only for cosmetics but also for various experimental purposes including muscle-related research. In this study, we applied BoNT/A to mouse muscle of three differ...

Authors: Min-Soo Seo, Young-In Kim, Kyung-Ku Kang, Se-Kyung Oh, Soo-Eun Sung, Young-Suk Jung, Joon Yong Cho, HyunKeun Song, Dae Youn Hwang, Sang-Joon Park and Kil Soo Kim

Comparative analysis of dose-dependent neurotoxic response to 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine in C57BL/6 N mice derived from three different sources

MPTP, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine is commonly used to induce nigrostriatal defects to induce parkinsonism and/or parkinsonian syndrome, to replicate the lesions seen in Parkinson’s disease (PD...

Authors: Dong-Joo Hwang, Ki-Chun Kwon, Hyun-Keun Song, Kil-Soo Kim, Young-Suk Jung, Dae-Youn Hwang and Joon-Yong Cho

Metabolomics approach to serum biomarker for laxative effects of red Liriope platyphylla in loperamide-induced constipation of SD rats

Red Liriope platyphylla (RLP) is a known herbal medicine used in the treatment of some chronic diseases including constipation, neurodegenerative disorders, diabetes and obesity. To determine and characterize put...

Authors: Ji Eun Kim, Young Ju Lee, Sung Ha Ryu, Ji Won Park, Mi Ju Kang, Hyeon Jun Choi, Su Ji Bae, Yusang Choi, Hyun Gu Kang, Kyu-Bong Kim, Suhkmann Kim, Yong Lim and Dae Youn Hwang

Aged mice show an increased mortality after anesthesia with a standard dose of ketamine/xylazine

Geriatric animal models are crucial for a better understanding and an improved therapy of age-related diseases. We observed a high mortality of aged mice after anesthesia with a standard dose of ketamine/xylaz...

Authors: Sandra Schuetze, Anja Manig, Sandra Ribes and Roland Nau

Reference values of hematological and biochemical parameters in young-adult cynomolgus monkey (Macaca fascicularis) and rhesus monkey (Macaca mulatta) anesthetized with ketamine hydrochloride

Nonhuman primate models are valuable in biomedical research. However, reference data for clinical pathology parameters in cynomolgus and rhesus monkeys are limited. In the present study, we established hematol...

Authors: Bon-Sang Koo, Dong-Ho Lee, Philyong Kang, Kang-Jin Jeong, Sangil Lee, Kijin Kim, Youngjeon Lee, Jae-Won Huh, Young-Hyun Kim, Sang-Je Park, Yeung Bae Jin, Sun-Uk Kim, Ji-Su Kim, Yeonghoon Son and Sang-Rae Lee

Tracking acute phase protein response during acute and chronic Toxoplasma gondii infection

Toxoplasmosis is a disease caused by the protozoan Toxoplasma gondii, which occurs worldwide in mammals and birds. Brain is the primary target organ because Toxoplasma gondii is a ubiquitous intracellular parasit...

Authors: Hasan Tarik Atmaca, Aycan Nuriye Gazyagci, Osman Safa Terzi, Gungor Cagdas Dincel and Tugce Sumer

Comparison of the virulence of Streptococcus pneumoniae in ICR mouse stocks of three different origins

Streptococcus pneumoniae causes many people to suffer from pneumonia, septicemia, and other diseases worldwide. To identify the difference in susceptibility of and treatment efficacy against S. pneumoniae in thre...

Authors: Jun-Young Kim, Sun-Min Seo, Han-Kyul Lee, Han-Woong Kim and Yang-Kyu Choi

Efficacy and local irritation evaluation of Eriobotrya japonica leaf ethanol extract

Although Eriobotrya japonica leaves have been studied as a raw material for various cosmetic products, little is known about the anti-oxidant, anti-inflammatory, and anti-melanogenic activities of Eriobotrya japo...

Authors: Nak-Won Seong, Won-Jun Oh, Il-Soo Kim, Su-Jin Kim, Ji-Eun Seo, Chang-Eon Park, Da-Young Kim, Je-Won Ko and Jong-Choon Kim

First comprehensive report of bacteria spp. associated with cloaca of Laudakia nupta (Sauria: Agamidae) in Iran using molecular studies

Iran bears a remarkable variety of reptiles. One of the lizard families occurring in Iran is the Family Agamidae which is widely are distributed throughout the old world. The large-scaled rock agamid, Laudakia nu...

Authors: Farkhondeh Sayyadi, Nasrullah Rastegar-Pouyani, Mehri Azadbakht and Khosrow Chehri

Evaluation of iNSiGHT VET DXA (Dual-Energy X-ray Absorptiometry) for assessing body composition in obese rats fed with high fat diet: a follow-up study of diet induced obesity model for 8 weeks

We examined the precision, accuracy, and capability of detecting changes of Dual-Energy X-ray Absorptiometry (DXA) for the measurements of total-body weight (TBW), total-body fat weight (TBFW), and total-body ...

Authors: Jungyun Yeu, Han-Jik Ko, Doyeun Kim, Youngbok Ahn, Jaejin Kim, Wonhee Lee, IlSin Jung, Jungkyu Suh and Seok-Jong Lee

Welcome to Laboratory Animal Research

Authors: Je Kyung Seong

Protective effects of cultured and fermented ginseng extracts against scopolamine-induced memory loss in a mouse model

Authors: Song-Hee Han, Sung-June Kim, Young Won Yun, Sang Yoon Nam, Hu-Jang Lee and Beom-Jun Lee

Genetic and morphometric characteristics of Korean wild mice (KWM/Hym) captured at Chuncheon, South Korea

Laboratory inbred mice are used widely and commonly in biomedical research, but inbred mice do not have a big enough gene pool for the research. In this study, genetic and morphometric analyses were performed ...

Authors: Hajin Nam, Yoo Yeon Kim, Boyoung Kim, Won Kee Yoon, Hyoung-Chin Kim and Jun Gyo Suh

CRISPR/Cas9-mediated knockout of CD47 causes hemolytic anemia with splenomegaly in C57BL/6 mice

CD47 (integrin-associated protein), a multi-spanning transmembrane protein expressed in all cells including red blood cells (RBCs) and leukocytes, interacts with signal regulatory protein α (SIRPα) on macropha...

Authors: Joo-Il Kim, Jin-Sung Park, Jina Kwak, Hyun-Jin Lim, Soo-Kyung Ryu, Euna Kwon, Kang-Min Han, Ki-Taek Nam, Han-Woong Lee and Byeong-Cheol Kang

Nucleotide-binding oligomerization domain 1 is dispensable for host immune responses against pulmonary infection of Acinetobacter baumannii in mice

Nucleotide-binding domain 1 (Nod1) is a cytosolic receptor that is responsible for the recognition of a bacterial peptidoglycan motif containing meso-diaminophimelic acid. In this study, we sought to identify the...

Authors: Min-Jung Kang, Jin-A Choi, Joo-Hee Choi, Ah-Ra Jang, Ji-Yeon Park, Jae-Hun Ahn, Tae-Sung Lee, Dong-Yeon Kim and Jong-Hwan Park

Inhibition of endoplasmic reticulum stress in high-fat-diet-induced obese C57BL/6 mice: Efficacy of a novel extract from mulberry (Morus alba) leaves fermented with Cordyceps militaris

A few clues about correlation between endoplasmic reticulum (ER) stress and mulberry (Morus alba) leaves were investigated in only the experimental autoimmune myocarditis and streptozotocin-induced diabetes. To i...

Authors: Mi Rim Lee, Su Ji Bae, Ji Eun Kim, Bo Ram Song, Jun Young Choi, Jin Ju Park, Ji Won Park, Mi Ju Kang, Hyeon Jun Choi, Young Whan Choi, Kyung Mi Kim and Dae Youn Hwang

Generation of knockout mouse models of cyclin-dependent kinase inhibitors by engineered nuclease-mediated genome editing

Cell cycle dysfunction can cause severe diseases, including neurodegenerative disease and cancer. Mutations in cyclin-dependent kinase inhibitors controlling the G1 phase of the cell cycle are prevalent in var...

Authors: Bo Min Park, Jae-il Roh, Jaehoon Lee and Han-Woong Lee

Disruption of the Tff1 gene in mice using CRISPR/Cas9 promotes body weight reduction and gastric tumorigenesis

Trefoil factor 1 (TFF1, also known as pS2) is strongly expressed in the gastrointestinal mucosa and plays a critical role in the differentiation of gastric glands. Since approximately 50% of all human gastric can...

Authors: Hyejeong Kim, Haengdueng Jeong, Yejin Cho, Jaehoon Lee, Ki Taek Nam and Han-Woong Lee

[¹⁸F]FET PET is a useful tool for treatment evaluation and prognosis prediction of anti-angiogenic drug in an orthotopic glioblastoma mouse model

O-2-¹⁸F-fluoroethyl-l-tyrosine ([¹⁸F]FET) has been widely used for glioblastomas (GBM) in clinical practice, although evaluation of its applicability in non-clinical research is still lacking. The objective of th...

Authors: Ok-Sun Kim, Jang Woo Park, Eun Sang Lee, Ran Ji Yoo, Won-Il Kim, Kyo Chul Lee, Jae Hoon Shim and Hye Kyung Chung

A comparison of metabolomic changes in type-1 diabetic C57BL/6N mice originating from different sources

Animal models have been used to elucidate the pathophysiology of varying diseases and to provide insight into potential targets for therapeutic intervention. Although alternatives to animal testing have been p...

Authors: Seunghyun Lee, Jae-Hwan Kwak, Sou Hyun Kim, Jieun Yun, Joon-Yong Cho, Kilsoo Kim, Daeyeon Hwang and Young-Suk Jung

Regulation of gastrointestinal hormones during laxative activity of gallotannin-enriched extract isolated from Galla Rhois in loperamide-induced constipation of SD rats

Regulation of gastrointestinal hormones have been reported in animal models for constipation undergoing laxative therapy when administered herbal products. We undertook to investigate whether the laxative acti...

Authors: Ji Eun Kim, Mi Ju Kang, Jun Young Choi, Jin Ju Park, Mi Rim Lee, Bo Ram Song, Hye Ryeong Kim, Ji Won Park, Hyeon Jun Choi, Su Ji Bae and Dae Youn Hwang

In vitro and in vivo inhibition of Helicobacter pylori by Lactobacilllus paracasei HP7

The efficacy of standard therapeutic strategies for Helicobacter pylori (H. pylori) infection is decreasing over time due to the emergence of drug-resistant strains. As an alternative, the present study investiga...

Authors: Seong-Soo Hong, Hyun-A Lee, Joo Yun Kim, Ji-Woong Jeong, Jae-Jung Shim, Jung Lyoul Lee, Jae-Hun Sim, Yungho Chung and Okjin Kim

Induction of superovulation in mature mice and rats using serum of spayed female dogs

The following experiments were designed to examine the effect of serum of spayed dogs on superovulation response in mice and rats. In Experiment 1, female mice at diestrus (n=30) were divided into three equal ...

Authors: M. H. Nooranizadeh, A. Mogheiseh, M. Kafi, M. Sepehrimanesh and H. Vaseghi

Repeated restraint stress promotes hippocampal neuronal cell ciliogenesis and proliferation in mice

Stress severely disturbs physiological and mental homeostasis which includes adult neurogenesis in hippocampus. Neurogenesis in hippocampus is a key feature to adapt to environmental changes and highly regulat...

Authors: Kyounghye Lee and Hyuk Wan Ko

Growing pigs developed different types of diabetes induced by streptozotocin depending on their transcription factor 7-like 2 gene polymorphisms

The different polymorphisms of the transcription factor 7-like 2 (TCF7L2) gene promote variances in diabetes susceptibility in humans. We investigated whether these genotypes also promote differences in diabet...

Authors: Ching-Fu Tu, Chi-Yun Hsu, Meng-Hwan Lee, Bo-Hui Jiang, Shyh-Forng Guo, Chai-Ching Lin and Tien-Shuh Yang

Mouse models of breast cancer in preclinical research

Breast cancer remains the second leading cause of cancer death among woman, worldwide, despite advances in identifying novel targeted therapies and the development of treating strategies. Classification of cli...

Authors: Mi Kyung Park, Chang Hoon Lee and Ho Lee

Crosstalk between FXR and TGR5 controls glucagon-like peptide 1 secretion to maintain glycemic homeostasis

Though bile acids have been well known as digestive juice, recent studies have demonstrated that bile acids bind to their endogenous receptors, including Farnesoid X receptor (FXR) and G protein-coupled bile a...

Authors: Hyeonhui Kim and Sungsoon Fang