Rodent models for psychiatric disorders: problems and promises

Table 1 Overview of the advantages, disadvantages, and references to promising genetic, neuroimaging, and neuromodulation technologies

	Advantages	Disadvantages	References
Genetics:
scRNA-seq	single cellular resolution	Expensive Large amount of data Improving bioinformatic pipelines	Kolodziejczyk et al. (2015) Ofengeim et al. (2017) Saliba et al. (2014)
CRISPR/Cas9	Cost and time efficient Precise editing Multiple genes simultaneously	Off-target effects Editing efficiency	Doudna & Charpentier (2014)
Neuroimaging:
Fiber Photometry	Cell-type specific High temporal resolution	Lack of individual cellular information	Dana et al. (2015) Li et al. (2019)
1/2-Photon Imaging	Cell-type specific High temporal resolution High spatial resolution	Larger head mount Limited free movement Widely invasive surgery	Leinweber et al. (2014) Ozbay et al. (2018)
Neuromodulation:
Optogenetics	Cell-type specific High temporal specificity Capability of fine temporal manipulation	Difficulty targeting deeper structures Implanted fiber cable	Boyden et al. (2005) Govorunova et al. (2015)
Chemogenetics	Cell-type specific Free moving behavior Higher penetrance Long duration effect	Potential off target effects of ligand Relative low temporal specificity	Armbruster et al. (2007) Magnus et al. (2019)

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