Fig. 5

Schematic diagram of (a) LSM principle in imaging zebrafish larvae through illumination and detection at a right angle. Illumination (excitation) focused in one direction through a cylindrical lens in which a thin section of the sample is illuminated vertically to the orientation of observation. Objective lens used for fluorescence detection perpendicularly to the sample. b LSM uses a planar illumination of the focal plane from the side (selective illumination) instead of a point illumination as in confocal microscopy (equal illumination), which enables LSM to capture images at a faster speed, reducing photodamage and offering optical sectioning compared to confocal microscopy