Table 2 IL2Rg knockout mice. 214 embryos were obtained from superovulated NOD/SCID female mice and 96 fertilized embryos were introduced with sgRNAs (1 and 2:40ng/ul) and Cas9 protein (40ng/ul). 45 embryos were survived and 38 embryos implanted into oviducts of two pseudopregnant female mice. 3 mutant mice were identified from 13 pups