Fig. 4
From: Early high-fat feeding improves histone modifications of skeletal muscle at middle-age in mice
Age-related changes of histone distributions in the tibialis anterior muscle. ChIP-qPCR analysis was performed using 3 muscle samples selected in each group that showed the typical expression pattern in gene expression analysis. Distributions of total H3, H3.3, pan-acetyl H3 (H3ac), H3 acetylated at lysine 27 (H3K27ac), H3 tri-methylated at lysine 27 (H3K27me3), and H3 mono-methylated at lysine 4 (H3K4me1) were compared by the locus and the age. The values of ChIP results (% input) obtained from Control and High fat groups were combined (n = 6 for each bar graph), averaged, and compared between 3- and 13-mo-old. Note that distribution of H3.3 elevated with age in all loci. *: p < 0.05 in 3- vs. 13-mo-old examined by one-way ANOVA. P, Pdk4; U, Ucp3; Z, Zmynd17