Table 2 Sequences of primer pairs for ChIP-qPCR analysis
From: Early high-fat feeding improves histone modifications of skeletal muscle at middle-age in mice
Gene symbol | Distance from transcription start site | Forward primer | Reverse Primer |
|---|---|---|---|
Pdk4 | −1.5 to − 1.0 kbp | CATTAGGCAGACCCCCAGTA | CATGAGAGCTCACCCAGTCA |
−1.0 to −0.5 kbp | CAAAGGCTGCTTTTTGGAAG | TGTGGTGGAAAATCCCAGTT | |
−0.5 to 0 kbp | TCCTAGCGACCTGGGATCTA | CTAGAAAGGCCTGGCACAGT | |
0 to + 0.5 kbp | GAGCTGTTCTCCCGCTACAG | CAGTCGTCCTAGCCCTGAAC | |
+ 0.5 to + 1.0 kbp | ACACACCCCGCTGATCTCT | AAGGAGACAGAGGCCTTTCC | |
+ 1.0 to + 1.5 kbp | CGCTTCACCCACTACAGGTT | TTCAAAACCGTGTGTCCTCA | |
Ucp3 | −1.5 to −1.0 kbp | AACTCCCAGCAGAGGTTTGA | TGCTTTGAGCTCAGTCTGGA |
−1.0 to −0.5 kbp | AGCATGTCCATGTCATGAGG | TAGCCTCTTGTGGCTGGTTT | |
−0.5 to 0 kbp | CGTCTTCTCCTCTCCCCTCT | GGTTTAGCTTCCGTGACCTG | |
0 to + 0.5 kbp | TACCCAACCTTGGCTAGACG | AAAACACGGGAGTTGTGAGG | |
+ 0.5 to + 1.0 kbp | TGTGGGTACCCCAGTAGAGC | CCCAAGTTTTGAAGGAGTGC | |
+ 1.0 to + 1.5 kbp | GGATTGACAGGGAAGCAAAA | TGTGTTCTGCTCTGCTTTGG | |
Zmynd17 | −1.5 to −1.0 kbp | CTCTGCTTGCTTCCTTCCTC | ATGGTGGCTCACAACCATTT |
−1.0 to −0.5 kbp | GGGAATCCTTTGGAATCCTT | CTTCATCCCCTACTCCCACA | |
−0.5 to 0 kbp | AGTGGGTCCTTTCCTTTGGT | CCAAAGTCAGGCAAGAGGAG | |
0 to + 0.5 kbp | CCAGGTCCTGACTTGTCTCG | TAAGCCCTAGGCTTCCTTCC | |
+ 0.5 to + 1.0 kbp | CTTCCCACCTTCATCCAAAA | ACCCTTCATCCCCAAAGTCT | |
+ 1.0 to + 1.5 kbp | GGTCTGGGTCTGTGATAGCC | GCAGCACACCACTTGAAAGA |