Fig. 4

F-J B positive cells in the somatosensory cortex of the ND/sham (A), ND/TFI (B-D), HFD/sham (E), and HFD/TFI (F-H) groups at 1 day, 2 days, and 5 days after TFI. F-J B positive cells in the ND/TFI group appear in layer III and V at 5 days after TFI, however, in the HFD/TFI group, F-J B positive neurons appear from 2 days after TFI. Note that numbers of F-J B positive cells are higher in the HFD/TFI group than ND/TFI group at 5 days after TFI. Scale bar = 200 μm (A–H), 30 μm (a–p). I Mean number of F-J B positive cells in layer III and V (n = 5 in each sham group, n = 7 in each TFI group; ∗P < 0.05 vs. each sham group, †P < 0.05 vs. pre-time point group, and #P < 0.05 vs. ND/TFI group). The bars indicate the means ± SEM