Fig. 2

A clinical-grade intracaecal surgery results in intracaecal tumour growth. a Surgery set-up in a biological safety cabinet. Surgery is performed on a microscope (i.) to visualise the space between the serosa and subserosa of the caecum. The mouse is anaesthetised in the induction box (ii.) before transfer to the nose cone, both of which are attached to gas scavengers (iii.) to remove waste gas. A hot bead steriliser (iv.) is used to sterilise instrument tips between mice. A non-sterile area (v.) contains pre-surgery prep items and a sterile area (vi.) contains tools and disposables for use during surgery. b Photo of in cabinet set-up. c Photos of developed intracaecal CT26 tumours on the caecum as indicated by arrows. Some mice grew multiple tumours, as indicated by multiple arrows. d Frequency of mice that received intracaecal CT26 cell injection which developed detectable tumours which could be analysed by flow cytometry, n = 15