Table 1 Primers used for quantitative real-time PCR
From: Comparative analysis of immune related genes between domestic pig and germ-free minipig
Gene symbol1 | Primer sequences (from 5′ to 3′) | Length (bp) | Gene Bank ID |
|---|---|---|---|
OAS1 | F: AGAGTCCACGACGGGAGAACC | 111 | MG799562.1 |
R: ACTGACCCAGGGCATCAAAGG | |||
CD40LG | F: ATTCACTTGGGCGGAGTCTTC | 80 | HQ110108.1 |
R: GTGGCTCACTTGGCTTGGAT | |||
IL-1B | F: GAAGTGATGGCTAACTACGGTGAC | 108 | NM_214055.1 |
R: TCTCAGAGAACCAAGGTCCAGGT | |||
IL-6 | F: AAAGAATCCAGACAAAGCCACC | 83 | NM_001252429.1 |
R: TCCACTCGTTCTGTGACTGCA | |||
IL-18R1 | F: ATGATTATGTTTTGGAGTTTT | 373 | NM_214098.1 |
R: GTAATATTGAAGGTTTTGGTGA | |||
GAPDH | F: GCTACACTGAGGACCAGGTTG | 294 | NM_001206359.1 |
R: AGGAGATGCTCGGTGTGTTG |