Fig. 2

Difference of doxorubicin efficacy between male- and female-derived xenograft models. a. Tumor growth curve. Hep3B cells were transplanted in male Balb/c nu/nu mice and SNU-387 cells were in female mice. Data are presented as mean ± standard deviation (n = 4/group). * p < 0.05 (Student t-test). b. Expression of Ki-67 in the tumor tissues, determined by immunohistochemistry. (Left panel) Brown dots indicate Ki-67 expression. Scale bar, 100 μm (Right panel). Brown dots were counted using ImageJ. Data are shown as mean ± standard deviation (n = 3/group). * p < 0.05 (Student t-test). c. Apoptosis-related protein expression in tumor tissues, determined by western blotting. (Cont, control; Dox, doxorubicin)