Fig. 6

Histopathological changes in the lungs of mice obtained from the OVA-PM model. A Experimental schedule. OVA or PBS were intraperitoneally injected on days 0, 7, and 14. Thereafter, ovalbumin or PBS were intranasally inoculated on days 21 and 22, followed by intratracheally injection of PM-b or PBS on day 23. All mice were euthanized on day 30 (CON group: n = 5; OVA group: n = 6; OVA+PM group: n = 5). B Representative images of the lungs of mice at the day of autopsy. Infiltration of inflammatory cells were observed in the OVA group and OVA+PM group (Red arrow head on the HE staining). The number of infiltrated cells were increased in the OVA+PM group, as compared to the OVA group. The number of PAS positive cells were greater in lungs obtained from the OVA+PM group than the OVA group (Red arrow on the PAS staining). Scale bar: 100 μm. C Serum total IgE of all mice was measured by ELISA and compared between groups. The bar graphs are presented as mean ± standard error of mean. One-way ANOVA test was used for statistical analysis. * p < 0.05, ** p < 0.01