Fig. 2
From: Loss-of-function mutation in Pcsk1 increases serum APOA1 level and LCAT activity in mice
Increased mature lipid-free APOA1 level in PCSK1N222D mice. a Top: increased serum total APOA1 level in PCSK1N222D mice, (n = 5–6/genotype); bottom: western blot images of a representative band for APOA1 by genotype. b Top: similar APOA1 level in NHDS fraction, (n = 4–5/genotype); bottom: western blot images of a representative band for APOA1 by genotype. c Top: increased mature/immature APOA1 level (n = 5–6/genotype); bottom: IEF images of a representative band for immature and mature forms of APOA1 by genotype. Samples were obtained from fasted 8-week-old PCSK1N222D and PCSK1WT males. Data represent the mean ± SEM from the number of animals of each group. Equal serum volume was loaded into each well. WB —western blot, IEF —iso-electro focusing *P < 0.05