Fig. 3
From: Loss-of-function mutation in Pcsk1 increases serum APOA1 level and LCAT activity in mice
Comparison of Apoa1 mRNA and APOA1 protein levels in liver of PCSK1WT and PCSK1N222D mice. a Similar liver Apoa1 mRNA level, measured by qPCR, (n = 5/genotype), b Top: slightly reduced liver APOA1 protein levels (n = 5/genotype); bottom: western blot images of a representative band for APOA1 and β-actin by genotype. Tissue samples were obtained from fasted 8-week-old male mice. Equal amount of protein was loaded into each well. Relative level of APOA1 was calculated using the intensity of APOA1 protein band normalized by β-actin band. Data represent the mean ± SEM from the number of animals of each group. WB —western blot