Table 3 Sequence of the primers used for PCR amplification
Gene | Primer sequences (F, forward; R, reverse) | Product (bp) |
|---|---|---|
Ubiquitin carboxy-terminal hydrolase L1 | F: 5′-TGAAGCAGACCATCGGGAAC-3′ R: 5′-GAGTCATGGGCTGCCTGAAT-3′ | 190 |
Ubiquitin thiolesterase OTUB1 | F: 5′-GCGACCACATCCACATCA-3′ R: 5′-ATGACCATTTACAACCACA-3′ | 292 |
Proteasome subunit alpha type 1 | F: 5′-CCAACACAGCGATATGGCCG-3′ R: 5′-CTCTCCAGGTAAGTGCGAGC-3′ | 167 |
Proteasome subunit alpha type 3 | F: 5′-GGCACTGGGTATGACCTGTC-3′ R: 5′-AAGGAACGAGCATCTGCCAA-3′ | 251 |
β-actin | F: 5′-TACAACCTTCTTGCAGCTCCTC-3′ R: 5′-CCTTCTGACCCATACCCACC-3′ | 205 |