Fig. 2

Effect of MCAo-induced cerebral ischemia and prolonged anesthesia on physiological activity and survival rate. A Alterations from baseline body weight to 24 h after MCAo. Body weight was measured before the MCAo and 72 h post-reperfusion. Data are presented as mean ± S.D. B Survival rate is defined as the percentage of surviving animals from induction of ischemia-reperfusion until ischemic brain injury assessment. Survival data were expressed by the Kaplan–Meier curve, and compared using the log-rank test. 5 out of 5, 10 out of 14, 18 out of 25, and 19 out of 25 animals survived in sham, control, ISO, and Xyl groups, respectively. Mortality data were recorded for 72 h to conduct a survival analysis. 5 (sham), 14 (control), 25 (ISO), and 25 (Xyl) animals were used in this study. Data are presented as mean ± S.D p-value was considered significant at *p < 0.005