Table 2 List of forward and reverse primers used for amplification
Gene | Primer sequences (F, Forward; R, Reverse) | Product (bp) |
|---|---|---|
Adenosylhomocysteinase | F: 5′-AAGCTGCCATGGAGGGCTAC3′ R: 5′-GATGGCAGCTGGAAGGTGAA-3′ | 583 |
Glycerol-3-phosphate dehydrogenase | F: 5′-GGCCCTTTCACAGACTCCGT-3′ R: 5′-TCCATGTTCTCGGGGCTGT-3′ | 117 |
Eukaryotic initiation factor 4A-II | F: 5′-CAGAGGGAATGGACCCCGAT-3′ R: 5′-GTGGCTGTCTTGCCAGTACC-3′ | 214 |
Apolipoprotein A-I | F: 5′-GGGAGTTCTGGCAGCAAGAT-3′ R: 5′-GCTGTTTGCCCAAAGTGGAG-3′ | 138 |
Mu-crystallin | F: 5′-TCCCAGCAATGGTTCCCTGC-3′ R: 5′-TATCACTGCCTGGGGGCTTC-3′ | 113 |
β-actin | F: 5′-TACAACCTTCTTGCAGCTCCTC-3′ R: 5′-CCTTCTGACCCATACCCACC-3′ | 205 |