Fig. 3

Alveolar bone loss during periodontitis progression. A Representative images of furcation and alveolar bone loss in mice with periodontitis. Alveolar bone loss was determined by the alveolar bone area in the furcation area (control group; D7 n = 7, D14 n = 7, D28 n = 10, and periodontitis group; D7 n = 7, D14 n = 9, D28 n = 11) and the CEJ-ABC distance in the distal area (control group; D7 n = 7, D14 n = 7, D28 n = 10, and periodontitis group; D7 n = 7, D14 n = 9, D28 n = 10). Scale bar = 200 μm. B Representative images and osteoclast formation in mice with periodontitis. Osteoclast formation was determined by counting TRAP-positive osteoclasts (control group; D7 n = 8, D14 n = 8, D28 n = 8, and periodontitis group; D7 n = 7, D14 n = 9, D28 n = 12). Black arrowheads indicate representative TRAP-positive osteoclasts. Scale bar = 200 μm. Data are presented as the mean ± SE. *p < 0.05 versus control. #p < 0.017 versus days. AB, alveolar bone; ABC, alveolar bone crest; CEJ, cementoenamel junction; No, number; TRAP, tartrate-resistant acid phosphatase