Table 1 Primers used for real-time PCR
Target | Primers (5′ to 3′) | Annealing Tm |
|---|---|---|
Runx2 | F: GCCTTCAAGGTGGTAGCCC R: TCCACTCCGGCCCACAAATC | 60 |
OCN | F: TAGTGAAGAGACCCAGGCGCTA R: TCACAGTCCGGATTGAGCTCA | 60 |
TRPC1 | F: ATCAAAAGGCAAGGTCAAACGG R: ACAGATCTTGGCGCAGTTCGT | 57 |
TRPC3 | F: GCATTCTCAATCAGCCAACACG R: TCCTCAGTTGCTTGGCTCTTGT | 57 |
TRPC4 | F: ATGAGGAACCTGGTGAAGCGATA R: GCATTCGCAGATTGTATTGTGGA | 54 |
TRPC6 | F: CATTTACTGGTTTGCTCCATGCA R: GTGCTGGTTTCATTAGGAAGGAG | 57 |
GAPDH | F: CAAATTCCATGGCACCGTCA R: GACTCCACGACGTACTCAGC | 58 |