Fig. 1
Design for Trp53 gene deletion, histopathology of solid tumor and Trp53 expression. A FVB/N-Trp53tm1Hw1 mice used in this study have the highly active TALENs specific to exon 2 of Trp53. B H&E stained sections of solid tumor from the FVB/N-Trp53tm1Hw1 mice were observed at 100 × (left column) and 400 × (right column) using a light microscope. Rectangles in the left column were magnified into the right column. C After UV radiation with 40 J, the cell homogenates were prepared from the primary tumor cells of the subset group, and transferred onto nitrocellulose membranes. The levels of Trp53 protein and β-actin were detected with specific antibodies, followed by horseradish peroxidase-conjugated goat anti-rabbit IgG. The intensity of each band was determined using an imaging densitometer. The data presented represents the means ± SD (n = 3). *, p < 0.05 relative to the No treated group. #, p < 0.05 relative to the CT26 cells