Fig. 1

Improved prognosis in NOD.SCID mice than CB17.SCID mice after IAV infection. (A) Body weight and (B) mortality were monitored in BALB/c, CB17.SCID, and NOD.SCID mice after infection with IAV. Cumulative data from three independent experiments (n = 15 per group) show attenuated weight loss and prolonged survival in NOD.SCID mice compared to the CB17.SCID control. Survival rate differences were statistically significant as determined by the log-rank (Mantel-Cox) test. (C) On day 5 post-infection, lungs were harvested from IAV-infected mice, and the virus titer was measured by plaque assay. Cumulative data from three mouse groups (n = 5 per group) show no difference in virus titers between the groups. (D–G) Immune cells were extracted from the lungs of CB17.SCID and NOD.SCID mice on days 1, 3, and 5 post-infection, with the absolute number of each immune cell type determined through flow cytometric analysis. Comparative evaluation of total cell counts (D), neutrophils (E), eosinophils (F), CCR2⁺ inflammatory monocytes (G), and alveolar macrophages (H) between the two groups was carried out. Cumulative data from two groups of mice (n = 5 per group) show statistically significant differences in immune cell counts, analyzed using an unpaired t-test. Statistical significance is indicated as follows: *p < 0.05; **p < 0.04; ***p < 0.001