Fig. 2

Comparative analysis of gene expression profiles and selected chemokine genes in IAV-infected lungs of CB17.SCID and NOD.SCID mice. Total mRNA was extracted from the lungs on day 1 post-infection, and bulk RNA sequencing analysis was conducted. (A) A heatmap depicting differentially expressed genes between CB17.SCID and NOD.SCID mice (n = 2 per group). Gene sets enriched in NOD.SCID (B) and CB17.SCID (C) mice were analyzed using Bioplanet 2019 with p-value ranking in Enrichr, showing the top 10 dominant pathways within each mouse group. Volcano plots showing differentially expressed cytokine (D) and chemokine (E) genes between the two groups. The transcript levels of each gene were measured by quantitative reverse transcription real-time PCR, and relative gene expression levels were calculated using Gapdh as a control gene. (F) Summarized results from two groups of mice (n = 7 per group) show statistically significant upregulation of chemokine genes in CB17.SCID lungs compared to those of NOD.SCID mice. (G) A summarized graph obtained from the same dataset as (F) shows higher expression of Cxcl14 chemokine genes in NOD.SCID lungs than in CB17.SCID mice on day 1 post-infection. Statistical significance indicated as follows: *p < 0.05; ***p < 0.001