Fig. 4

Comparsion of innate cells, cytokines, and virus titers in IAV-infected lungs of naive and AAV9-EGFP vector-treated mice. Cells were isolated from mouse lungs before IAV infection (D0) or on days 2 (D2) and 4 (D4) post-infection, four weeks after AAV9-EGFP vector administration. Absolute numbers of each immune cell were calculated based on flow cytometry analysis: CD45.2⁺ cells (A), trans-macrophages (B), and neutrophils (C). Naive mice infected without pre-administered AAV9 vector were used as controls. Cumulative results from two independent experiments (n = 5–10 per group) show lower numbers of neutrophils and trans-macrophages on day 4 post-infection in the lungs of AAV9 vector-treated mice compared to naive controls. Total mRNA was extracted from mouse lungs before IAV infection (D0) or on days 2 (D2) and 4 (D4) post-infection, four weeks after AAV9-EGFP vector administration. (D) IFN- β gene expression levels were quantified by qPCR and normalized to Gapdh. Cumulative results from two independent experiments (n = 5–10 per group) showed no significant difference between the two groups. (E) Total mRNAs were extracted from the lungs of mice infected with IAV in the presence or absence of pre-administered AAV9 vector. Levels of IAV N gene transcripts were measured by quantitative PCR, with Gapdh as a control gene for relative expression. Cumulative results from two independent experiments (n = 5–10 per group) show no difference in IAV gene expression levels in between the two groups. The data are means ± SDs; the x-values are fold changes. Statistical significance is indicated as follows: *p < 0.05