Fig. 2

FMC-EV enhanced liver metastasis of FMC in FMC-xenograft-bearing mice. A Growth of FMC-1807-xenograft in mice injected with FMC-EV or PBS was measured weekly with a caliper. n = 6 in each group. B, C H&E staining (left panel) of the lung taken from a FMC-bearing mouse injected with PBS (B) or with FMC-EV (C). Vimentin expression (brown) in metastasized FMC in lung was analyzed by IHC (right panel). D The FMC foci in the lung were quantified by counting the number of foci which were vimentin-positive and presented as a ratio of the area of FMC foci to that of the entire lung section (E). F, G H&E staining (left panel) of the liver taken from an FMC-bearing mouse injected with PBS (F) or with FMC-EV (G). Vimentin expression in metastasized FMC in liver was analyzed by IHC (right panel). H The FMC foci in the liver were quantified by counting the number of foci which were vimentin-positive and presented a ratio of the area of FMC foci to that of the entire liver section (I). The statistical significance between groups was determined using the Student’s t-test