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Fig. 6 | Laboratory Animal Research

Fig. 6

From: Feline mammary carcinoma-derived extracellular vesicle promotes liver metastasis via sphingosine kinase-1-mediated premetastatic niche formation

Fig. 6

Depletion of SK1 impaired cargo sorting in FMC-EV. A Depletion of SK1 expression in two SK1-knockout (SK1-KO) clones (C1 and C4) derived from FMC-1807 was verified by Western blotting analysis. B The concentration and size distribution of SK1-KO EV (right panel) were comparable with that of FMC-EV (left panel). Representative data of nanoparticle tracking analysis are shown. C Semi-quantitation of EV content by flow cytometric analysis. Bead-coupled EV was stained with primary antibodies specific to CD44, VDAC1, ANXA2, or MIF, followed by incubation with appropriate PE-conjugated secondary antibodies

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Laboratory Animal Research

ISSN: 2233-7660