Fig. 2

Tissue distribution of TH in the substantia nigra (A) and striatum (B) of NSE-hαSyn Tg mice. After staining specific antibodies, the stained brain section was observed by optical microscopy at 100x and 400x magnification. Brown or dark brown indicates the expression of TH in the cytoplasm of the substantia nigra and striatum. The immunostained slides were prepared from three to five mice per group, and the color density for each sample were observed in duplicate. Abbreviations: Abbreviation; hαSyn human α synuclein, TH tyrosine hydroxylase, NSE neuron-specific enolase, Tg transgenic mice, ML medial lemniscus, VTA ventral tegmental area, SNc substantia nigra pars compacta, LV lateral ventricle, St striatum, Cx cortex, cc corpus callosum