Table 2 Biochemical estimation data of compounds 28, 28a–e
Compound | % Inhibition AChE | LPO assay, MDA levels (Nm/mg of tissue) | Nitrite assay, nitrite conc. (Μm/mg) protein | GSH assay, μmol of GSH/mg Protein | SOD assay, SOD units/mg protein | Catalase assay, catalase activity min−1 |
|---|---|---|---|---|---|---|
Control | – | 16.58 ± 0.04 | 17.88 ± 0.05 | 0.02 ± 0.001 | 4.60 ± 0.03 | 11.66 ± 0.05 |
Scp + vehicle | – | 25.09 ± 0.03 | 31.21 ± 0.04 | 0.08 ± 0.002 | 0.78 ± 0.01 | 2.25 ± 0.04 |
Scp + donepezil | 46.6 ± 0.04 | 16.76 ± 0.04 | 16.63 ± 0.05 | 0.18 ± 0.002 | 3 ± 0.03 | 10.3 ± 0.05 |
Scp + 28 | 72.5 ± 0.04 | 8.50 ± 0.04 | 9.80 ± 0.03 | 0.3 ± 0.005 | 4.8 ± 0.04 | 5.28 ± 0.03 |
Scp + 28a | 45 ± 0.04 | 4.08 ± 0.04 | 17.82 ± 0.08 | 0.09 ± 0.003 | 3.13 ± 0.05 | 4.05 ± 0.05 |
Scp + 28b | 60.8 ± 0.05 | 3.13 ± 0.05 | 10.64 ± 0.04 | 0.09 ± 0.005 | 4.08 ± 0.04 | 4.56 ± 0.04 |
Scp + 28c | 34.3 ± 0.04 | 3.07 ± 0.02 | 12.96 ± 0.04 | 0.1 ± 0.001 | 3.07 ± 0.02 | 4.62 ± 0.05 |
Scp + 28d | 62.5 ± 0.05 | 4.09 ± 0.04 | 9.80 ± 0.03 | 0.2 ± 0.004 | 4.19 ± 0.04 | 5.14 ± 0.05 |
Scp + 28e | 57 ± 0.03 | 3.14 ± 0.04 | 13.32 ± 0.02 | 0.08 ± 0.001 | 3.14 ± 0.04 | 4.71 ± 0.04 |