Fig. 3
The Results of immunohistochemical of NAG, TNF-α, MPO expression and TUNEL assay. A, B, C NAG, D, E, F TNF-α, G, H, I, J MPO. K, L, M, N TUNEL and A, D, G, K CON group, B, E, H, L VAN group, C, F, I, M GEB group, J, N positive cell counting results. As a result of NAG, the CON group was showed little expression of NAG in the cytoplasm of the proximal tubule. In the VAN group, there was strong and more expression of NAG (arrows) compared to CON group. The GEB group was decreased and weaken expression of NAG than VAN group. As a result of TNF-α, the CON group was showed low expression of TNF-α in the cytoplasm of the proximal tubule. In the VAN group, there was strong and more expression of TNF-α (arrows) compared to CON group. The GEB group was decreased and weaken expression of TNF-α than VAN group. As a result of MPO, the VAN group was increased MPO positive cells than CON group. In the GEB group, there was reduced MPO positive cells (arrows) than VAN group. As a result of TUNEL assay, the VAN group was increased TUNEL positive cells compared to the CON group. In the GEB group, there was reduced TUNEL positive cells (arrows) compared to the VAN group. The GEB group was decreased and weaken expression of NAG than VAN group. Scale bars in A-F are 200μm, and in G-M 400μm, each. * vs CON group (p < 0.01) # vs VAN group (p < 0.01)