Skip to main content
Download PDF

Effectiveness of the combinational treatment of Laminaria japonica and Cistanche tubulosa extracts in hair growth

Laboratory Animal Research volume 31, pages 24–32 (2015)Cite this article

Abstract

Since scalp hair loss has increased recently even in young people, seriously affecting individual’s quality of life, the hair growth-stimulating effects of Laminaria japonica extract (LJE) and Cistanche tubulosa extract (CTE) were investigated. After confirming anagen phase of follicles under shaving, male C57BL/6 mice were dermally applied with 3% Minoxidil or orally administered with the combinations of LJE and CTE for 21 days. Minoxidil promoted the hair regrowth and increased γ-glutamyl transpeptidase (γ-GTP) and alkaline phosphatase (ALP) activities. In addition, Minoxidil up-regulated epidermal growth factor (EGF) and vascular endothelial growth factor (VEGF) levels. Co-administration of LJE and CTE at 54 mg/kg LJE plus 162 mg/kg CTE exerted synergistic promoting effects on the hair regrowth, comparable to 3% Minoxidil. LJE preferentially enhanced ALP activity, while CTE increased both γ-GTP and ALP activities as well as EGF and VEGF expressions. In vivo air pouch inflammation model, carrageenan-induced vascular exudation and increased nitric oxide and prostaglandin E2 concentrations in the exudates were synergistically suppressed by co-administration of LJE and CTE. In addition, inflammatory cell infiltration was substantially inhibited by the combinational treatment. The results suggest that combinational oral treatment with LJE and CTE in appropriate doses and ratios prevent hair loss and improve alopecia, which might be in part mediated by their anti-inflammatory activities.

References

  1. Kim JS, Kang BS, Park HJ, Kim DY, Hwang SY, Nam SY, Yun YW, Lee BJ. Promoting effect of herbal extracts mixtures on hair regrowth in C3H/HeJ mice. J Biomed Res 2009; 10(3): 99–108.

    Google Scholar 

  2. Paus R, Cotsarelis G. The biology of hair follicles. N Engl J Med 1999; 341(7): 491–497.

    Article  CAS  PubMed  Google Scholar 

  3. Cotsarelis G. The hair follicle: dying for attention. Am J Pathol 1997; 151(6): 1505–1509.

    CAS  PubMed  PubMed Central  Google Scholar 

  4. Cotsarelis G, Sun TT, Lavker RM. Label-retaining cells reside in the bulge area of pilosebaceous unit: implications for follicular stem cells, hair cycle, and skin carcinogenesis. Cell 1990; 61(7): 1329–1337.

    Article  CAS  PubMed  Google Scholar 

  5. Oshima H, Rochat A, Kedzia C, Kobayashi K, Barrandon Y. Morphogenesis and renewal of hair follicles from adult multipotent stem cells. Cell 2001; 104(2): 233–245.

    Article  CAS  PubMed  Google Scholar 

  6. Burton JL, Marshall A. Hypertrichosis due to minoxidil. Br J Dermatol 1979; 101(5): 593–595.

    Article  CAS  PubMed  Google Scholar 

  7. Meisheri KD, Cipkus LA, Taylor CJ. Mechanism of action of minoxidil sulfate-induced vasodilation: a role for increased K+ permeability. J Pharmacol Exp Ther 1988; 245(3): 751–760.

    CAS  PubMed  Google Scholar 

  8. Buhl AE, Waldon DJ, Baker CA, Johnson GA. Minoxidil sulfate is the active metabolite that stimulates hair follicles. J Invest Dermatol 1990; 95(5): 553–557.

    Article  CAS  PubMed  Google Scholar 

  9. Kaufman KD, Dawber RP. Finasteride, a Type 2 5alpha-reductase inhibitor, in the treatment of men with androgenetic alopecia. Expert Opin Investig Drugs 1999; 8(4): 403–415.

    Article  CAS  PubMed  Google Scholar 

  10. Feldman SC, Reynaldi S, Stortz CA, Cerezo AS, Damont EB. Antiviral properties of fucoidan fractions from Leathesia difformis. Phytomedicine 1999; 6(5): 335–340.

    Article  CAS  PubMed  Google Scholar 

  11. Wang J, Zhang Q, Zhang Z, Song H, Li P. Potential antioxidant and anticoagulant capacity of low molecular weight fucoidan fractions extracted from Laminaria japonica. Int J Biol Macromol 2010; 46(1): 6–12.

    Article  CAS  PubMed  Google Scholar 

  12. Bojakowski K, Abramczyk P, Bojakowska M, Zwoliñska A, Przybylski J, Gaciong Z. Fucoidan improves the renal blood flow in the early stage of renal ischemia/reperfusion injury in the rat. J Physiol Pharmacol 2001; 52(1): 137–143.

    CAS  PubMed  Google Scholar 

  13. Li N, Zhang Q, Song J. Toxicological evaluation of fucoidan extracted from Laminaria japonica in Wistar rats. Food Chem Toxicol 2005; 43(3): 421–426.

    Article  PubMed  CAS  Google Scholar 

  14. Shimoda H, Tanaka J, Takahara Y, Takemoto K, Shan SJ, Su MH. The hypocholesterolemic effects of Cistanche tubulosa extract, a Chinese traditional crude medicine, in mice. Am J Chin Med 2009; 37(6): 1125–1138.

    Article  CAS  PubMed  Google Scholar 

  15. Yamada P, Iijima R, Han J, Shigemori H, Yokota S, Isoda H. Inhibitory effect of acteoside isolated from Cistanche tubulosa on chemical mediator release and inflammatory cytokine production by RBL-2H3 and KU812 cells. Planta Med 2010; 76(14): 1512–1518.

    Article  CAS  PubMed  Google Scholar 

  16. Kyung J, Kim D, Park D, Yang YH, Choi EK, Lee SP, Kim TS, Lee YB, Kim YB. Synergistic anti-inflammatory effects of Laminaria japonica fucoidan and Cistanche tubulosa extract. Lab Anim Res 2012; 28(2): 91–97.

    Article  PubMed  PubMed Central  Google Scholar 

  17. Chee E, Fong KS, Al Jajeh I, Nassiri N, Rootman J. The association of lacrimal gland inflammation with alopecia areata. Orbit 2015; 34(1): 45–50.

    Article  PubMed  Google Scholar 

  18. Brzezinska-Wcislo L, Bergler-Czop B, Wcislo-Dziadecka D, Lis-Swiety A. New aspects of the treatment of alopecia areata. Postepy Dermatol Alergol 2014; 31(4): 262–265.

    Article  PubMed  PubMed Central  Google Scholar 

  19. Hattori M, Ogawa H. Biochemical analysis of hair growth from the aspects of aging and enzyme activities. J Dermatol 1983; 10(1): 45–54.

    Article  CAS  PubMed  Google Scholar 

  20. Lee SH, Lee HJ, Baek IJ, Yon JM, Nam SY, Lee BJ, Yun YW, Kim YB, Hwang SY, Hong JT. Effect of DREAMMO® scalp lotion on hair growth promotion in an alopecia model of C57BL/6 mice. Lab Anim Res 2006; 22(1): 91–96.

    Google Scholar 

  21. Handjiski BK, Eichmüller S, Hofmann U, Czarnetzki BM, Paus R. Alkaline phosphatase activity and localization during the murine hair cycle. Br J Dermatol 1994; 131(3): 303–310.

    Article  CAS  PubMed  Google Scholar 

  22. Cross MJ, Dixelius J, Matsumoto T, Claesson-Welsh L. VEGFreceptor signal transduction. Trends Biochem Sci 2003; 28(9): 488–494.

    Article  CAS  PubMed  Google Scholar 

  23. Shin S, Jeon JH, Park D, Jang JY, Joo SS, Hwang BY, Choe SY, Kim YB. Anti-inflammatory effects of an ethanol extract of Angelica gigas in a Carrageenan-air pouch inflammation model. Exp Anim 2009; 58(4): 431–436.

    Article  CAS  PubMed  Google Scholar 

  24. Kim D, Park D, Kyung J, Yang YH, Choi EK, Lee YB, Kim HK, Hwang BY, Kim YB. Anti-inflammatory effects of Houttuynia cordata supercritical extract in carrageenan-air pouch inflammation model. Lab Anim Res 2012; 28(2): 137–140.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Rebora A. Pathogenesis of androgenetic alopecia. J Am Acad Dermatol 2004; 50(5): 777–779.

    Article  PubMed  Google Scholar 

  26. Shirai A, Ikeda J, Kawashima S, Tamaoki T, Kamiya T. KF19418, a new compound for hair growth promotion in vitro and in vivo mouse models. J Dermatol Sci 2001; 25(3): 213–218.

    Article  CAS  PubMed  Google Scholar 

  27. Kawabe TT, Kubicek MF, Johnson GA, Buhl AE. Use of gammaglutamyl transpeptidase activity as a marker of hair cycle and anagen induction in mouse hair follicles. J Invest Dermatol 1994; 103(1): 122–126.

    Article  CAS  PubMed  Google Scholar 

  28. Hamada K, Suzuki K. Evaluation of biochemical indices as a hair cycle marker in C3H mice. Exp Anim 1996; 45(3): 251–256.

    Article  CAS  PubMed  Google Scholar 

  29. Park SJ, Lee KW, Lim DS, Lee S. The sulfated polysaccharide fucoidan stimulates osteogenic differentiation of human adiposederived stem cells. Stem Cells Dev 2012; 21(12): 2204–2211.

    Article  CAS  PubMed  Google Scholar 

  30. Danilenko DM, Ring BD, Pierce GF. Growth factors and cytokines in hair follicle development and cycling: recent insights from animal models and the potentials for clinical therapy. Mol Med Today 1996; 2(11): 460–467.

    Article  CAS  PubMed  Google Scholar 

  31. Ozeki M, Tabata Y. In vivo promoted growth of mice hair follicles by the controlled release of growth factors. Biomaterials 2003; 24(13): 2387–2394.

    Article  CAS  PubMed  Google Scholar 

  32. Fujie T, Katoh S, Oura H, Urano Y, Arase S. The chemotactic effect of a dermal papilla cell-derived factor on outer root sheath cells. J Dermatol Sci 2001; 25(3): 206–212.

    Article  CAS  PubMed  Google Scholar 

  33. Stenn KS, Paus R. Controls of hair follicle cycling. Physiol Rev 2001; 81(1): 449–494.

    Article  CAS  PubMed  Google Scholar 

  34. Chapman RE, Hardy MH. Effects of intradermally injected and topically applied mouse epidermal growth factor on wool growth, skin and wool follicles of merino sheep. Aust J Biol Sci 1988; 41(2): 261–268.

    Article  CAS  PubMed  Google Scholar 

  35. Dvorak HF, Brown LF, Detmar M, Dvorak AM. Vascular permeability factor/vascular endothelial growth factor, microvascular hyperpermeability, and angiogenesis. Am J Pathol 1995; 146(5): 1029–1039.

    CAS  PubMed  PubMed Central  Google Scholar 

  36. Ellis RA, Moretti G. Vascular patterns associated with categen hair follicles in the human scalp. Ann N Y Acad Sci 1959; 83: 448–457.

    Article  CAS  PubMed  Google Scholar 

  37. Stenn KS, Fernandez LA, Tirrell SJ. The angiogenic properties of the rat vibrissa hair follicle associate with the bulb. J Invest Dermatol 1988; 90(3): 409–411.

    Article  CAS  PubMed  Google Scholar 

  38. Yano K, Brown LF, Detmar M. Control of hair growth and follicle size by VEGF-mediated angiogenesis. J Clin Invest 2001; 107(4): 409–417.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  39. Lachgar S, Charveron M, Ceruti I. Distribution of VEGF mRNA indifferent hair follicle stages by fluorescence in situ hybridization combined with confocal laser microscopy. In: Hair Research for the Next Millennium (Van Neste DJJ, ed), Elsevier, Amsterdam, 1996; pp 407–412.

    Google Scholar 

  40. Freyschmidt-Paul P, Sundberg JP, Happle R, McElwee KJ, Metz S, Boggess D, Hoffmann R. Successful treatment of alopecia areata-like hair loss with the contact sensitizer squaric acid dibutylester (SADBE) in C3H/HeJ mice. J Invest Dermatol 1999; 113(1): 61–68.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Author notes

  1. These two authors equally contributed to this work.

Authors and Affiliations

  1. College of Veterinary Medicine, Chungbuk National University, 1 Chungdaero (Gaesin-dong), Cheongju, Chungbuk, 362-763, Korea

    Kyungha Shin, Jangbeen Kyung, Dajeong Kim, Dongsun Park, Ehn-Kyoung Choi & Yun-Bae Kim

  2. Misuba RTech Co., Ltd., Asan, Korea

    Tae-Su Kim, Sung-Pyo Lee & Woong-Suk Yang

  3. Department of Food Science and Nutrition, Hoseo University, Asan, Korea

    Myung-Hwa Kang

Authors
  1. Kyungha Shin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tae-Su Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jangbeen Kyung
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Dajeong Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Dongsun Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Ehn-Kyoung Choi
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Sung-Pyo Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Woong-Suk Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Myung-Hwa Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Yun-Bae Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yun-Bae Kim.

Rights and permissions

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Shin, K., Kim, TS., Kyung, J. et al. Effectiveness of the combinational treatment of Laminaria japonica and Cistanche tubulosa extracts in hair growth. Lab Anim Res 31, 24–32 (2015). https://doi.org/10.5625/lar.2015.31.1.24

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.5625/lar.2015.31.1.24

Keywords

Laboratory Animal Research

ISSN: 2233-7660