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Comparative analysis of anti-Helicobacter pylori activities of FEMY-R7 composed of Laminaria japonica and Oenothera biennis extracts in mice and humans

Laboratory Animal Research volume 31, pages 7–12 (2015)Cite this article

Abstract

Helicobacter pylori-eliminating effects of FEMY-R7, composed of Laminaria japonica and Oenothera biennis extracts, were investigated in mice and humans. Male C57BL/6 mice were infected with the bacteria by intragastric inoculation (1×109 CFU/mouse) 3 times at 2-day intervals, and simultaneously, orally treated twice a day with total 20, 64 or 200 mg/kg/day FEMY-R7 for 2 weeks. In Campylobcter-like organism (CLO)-detection tests on gastric mucosa and feces, FEMY-R7 reduced the urease-positive reactivity in a dose-dependent manner; i.e., the positivity ratios were decreased to 70, 20, and 10% for gastric mocosa and to 80, 50, and 20% for feces. In a clinical sudy, human subjects, confirmed to be infected with Helicobacter pylori, were orally administered twice a day with capsules containing total 100, 320 or 1,000 mg/man/day FEMY-R7 (matching doses for 20, 64 or 200 mg/kg/day, respectively, in mice from a body surface area-based dose translation) for 8 weeks. FEMY-R7 decreased the positivity ratios in feces to 70, 40, and 30%, respectively. In bacterial culture, H. pylori was identified from the CLO-positive stools of mice and humans. The bacterial identification ratios exhibited a good correlation between the matching doses in mice and humans. It is suggested that FEMY-R7 could be a promising functional food without tolerance as an adjunct to reduce the dosage of antibiotics for the treatment of recurrent H. pylori infection.

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Author notes

  1. These two authors contributed equally to this work.

Authors and Affiliations

  1. Misuba RTech Co., Ltd., Asan, Korea

    Tae-Su Kim & Sung-Pyo Lee

  2. College of Veterinary Medicine, Chungbuk National University, 1 Chungdaero (Gaesin-dong), Cheongju, Chungbuk, 362-763, Korea

    Kyungha Shin, Ehn-Kyoung Choi, Youngjin Choi & Yun-Bae Kim

  3. Haewon Biotech Inc., Seoul, Korea

    Joseph H. Jeon

  4. Central Research Institute, Dr. Chung’s Food Co. Ltd., Chungbuk, Korea

    Yoon-Bok Lee

Authors
  1. Tae-Su Kim
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  2. Kyungha Shin
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Correspondence to Yun-Bae Kim.

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Kim, TS., Shin, K., Jeon, J.H. et al. Comparative analysis of anti-Helicobacter pylori activities of FEMY-R7 composed of Laminaria japonica and Oenothera biennis extracts in mice and humans. Lab Anim Res 31, 7–12 (2015). https://doi.org/10.5625/lar.2015.31.1.7

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  • DOI: https://doi.org/10.5625/lar.2015.31.1.7

Keywords

  • Helicobacter pylori
  • FEMY-R7
  • Laminaria japonica
  • Oenothera biennis
  • Campylobcter-like organism-detection test
  • bacterial identification

Laboratory Animal Research

ISSN: 2233-7660